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大黄素对人肾成纤维细胞增殖的影响
引用本文:宁英远,王俭勤,屈遂林.大黄素对人肾成纤维细胞增殖的影响[J].中国中西医结合杂志,2000,20(2):105-106.
作者姓名:宁英远  王俭勤  屈遂林
作者单位:兰州医学院附属第二医院!兰州730030(宁英远),中山医科大学(王俭勤),华西医科大学附属第一医院(屈遂林)
摘    要:目的:研究大黄素对人肾成纤维细胞增殖的影响。方法:在体外人肾成纤维细胞培养的基础上,采用^3H-TdR掺入法观察了不同浓度大黄素(10、30、50、80、100ug/ml)对人肾成纤维细胞^3H-TdR掺入率的影响。同时,利用流式细胞仪观察了大黄素(10、50、100ug/ml)对细胞周期的影响。结果:大黄素以剂量信赖方式降低了 人肾成纤维细胞^H-TdR掺入率(r=0.995,P〈0.01)。大黄素(50和1

关 键 词:大黄素  肾间质  成纤维细胞增殖  临床应用

Effect of Emodin on Human Kidney Fibroblast Proliferation
Authors:NING Yingyuan
Abstract:OBJECTIVE: To study the effect of emodin on proliferation of human kidney fibroblasts in vitro. METHODS: After human kidney fibroblasts were cultured, isolated and identified, both the effects of five different concentrations of emodin (10, 30, 50, 80 and 100 micrograms/ml) on 3H-TdR incorporation, and the effects of three different concentrations of emodin (10, 50 and 100 micrograms/ml) on cell cycle by flow cytometry were investigated. RESULTS: The exposure of human kidney fibroblasts to emodin (10-100 micrograms/ml) caused a dose-dependent reduction in 3H-TdR (r = 0.995, P < 0.01), and could delay the progress of human kidney fibroblasts from G1 to S phase. CONCLUSIONS: Emodin inhibited the proliferation of human kidney's fibroblasts by inhibiting the cell DNA synthase and delaying the progress of cell cycle. These findings might provide part of experimental basis for the clinical use of emodin.
Keywords:emodin  renal interstitial tissue  fibroblast  cell proliferation
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