补肾与活血复方调节老年鼠T细胞凋亡的对比研究

目的：研究老年肾虚Ｔ细胞功能减退的机理以及补肾延缓衰老的免疫学基础。方法：采用电镜，ＤＮＡ凝胶电泳及ＴＵＮＥＬ标记的流式细胞检测技术，对各组大鼠抗ＣＤ３单抗激活诱导的Ｔ细胞凋亡进行定性，定量分析。结果：老年组激活诱导的Ｔ细胞凋亡百分率为４７．０％，年轻组为２２．２％；补肾组为３１．２％，明显低于老年组。

Comparative Study of the Regulation of Kidney Tonic and Promoting Blood Circulation Recipe on Apoptosis in T Cells of Old Rats

Objective: To investigate the mechanism responsible for the observed reduction in T cell function of the Kidney Deficiency Syndrome. Methods: After 18th activation with anti CD 3 mAb, the qualitative and quantitative analysis of apoptoses in T cells were performed by using DNA gel electrophoresis, TdT mediated dUTP nick end labelling (TUNEL) and flow cytometry. Results: The percentage of apoptotic cells was 47.0% in the old and 22.2% in the young group (P<0 01). In the Kidney tonic group, the mean percentage of apoptotic cells was 31.2%, significantly lower than that in the aged group (P<0 05). The mean percentage of apoptotic cells in the promoting blood circulation group was 43.3% and no significant difference was seen in the percentages of apoptotoic T cells from the promoting blood circulation group compared with the old group. Conclusion: Activation induced excessive apoptosis of T cells is one of the mechanisms elucidating decreased T cell function of the Kidney Deficiency Syndrome and aging, the retardation of immune senescence by Kidney tonic recipe could be accounted for by down regulation of activation induced apoptosis in T cells.