电针联合复方丹参片对慢性脑缺血大鼠海马CA1区脑源性神经营养因子和血管内皮生长因子表达的影响

目的观察电针联合复方丹参片对慢性脑缺血大鼠海马CA1区脑源性神经营养因子(brain derived neurotrophic factor,BDNF)及血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法将50只SD大鼠随机分为正常对照组、模型组、复方丹参组、电针组和针药结合组,每组10只。以永久性双侧颈总动脉结扎术制备慢性脑缺血模型。造模后1周,复方丹参组和针药结合组大鼠给予复方丹参片0.75g/kg灌胃,每天1次,连续5周;电针组和针药结合组大鼠给予百会穴、大椎穴电针刺激,持续30min,每天1次,连续5周。采用免疫组织化学染色法结合图像分析检测大鼠海马CA1区BDNF及VEGF的表达。结果与正常对照组比较,模型组大鼠海马CA1区BDNF、VEGF阳性神经元数目及表达强度均明显减少(P<0.01);与模型组比较,复方丹参组、电针组和针药结合组海马CA1区BDNF、VEGF阳性神经元数目及表达强度均增加(P<0.01,P<0.05);针药结合组阳性神经元的数目和表达强度明显高于复方丹参组和电针组(P<0.01)。结论电针与复方丹参片结合可显著增加慢性脑缺血大鼠海马CA1区BDNF、VEGF的表达,且作用优于单用复方丹参片或电针。

Effects of Electroacupuncture Combined with Compound Salviae Miltiorrhizae Tablet on the Expressions of Brain Derived Neurotrophic Factor and Vascular Endothelial Growth Factor in Hippocampus CA1 of Chronic Cerebral Ischemia Rats

Objective To observe the effects of electroacupuncture(EA)combined with Compound Salviae Miltiorrhizae Tablet(CSMT)on the expressions of brain derived neurotrophic factor(BDNF)and vascular endothelial growth factor(VEGF)in hippocampus CA1 of chronic cerebral ischemia(CCI)rats.MethodsTotally 50 Sprague-Dawley rats were randomly divided into the normal control group,the model group,the CSMT group,the EA group,and the EA+CSMT groups,10 in each group.The CCI model was prepared by permanent bilateral common carotid arteries occlusion.One week after modeling,CSMT at 0.75 g/kg was given to rats in the EA+CSMT group and the CSMT group by gastrogavage,once daily,for 5 successive weeks.EA at Baihui(DU20)and Dazhui(DU14)was performed to rats in the EA group and the EA+CSMT group,lasting for 30 min,once daily,for 5 successive weeks.The expressions of BDNF and VEGF in the hippocampus CA1 area were detected by immunohistochemical assay and image analysis.Results Compared with the normal control group,the number of positively expressed BDNF and VEGF neurons and their expression intensity in the hippocampus CA1 of the model group obviously decreased(P<0.01).Compared with the model group,the number of positively expressed BDNF and VEGF neurons and their expression intensity in the hippocampus CA1 of the CSMT group,the EA group,and the EA+CSMT groups obviously increased(P<0.01,P<0.05).The number of positively expressed BDNF and VEGF neurons and their expression intensity were obviously higher in the EA+CSMT group than those of the CSMT group and the EA group(P<0.01).Conclusions EA combined with CSMT could significantly increase the expressions of BDNF and VEGF in the hippocampus CA1 of CCI rats.Besides,their effects were significantly higher than those of the CSMT group or the EA group.