氧化苦参碱对慢性乙型肝炎患者血清细胞因子及肝纤维化指标的影响

目的 探讨氧化苦参碱对慢性乙型肝炎患者血清细胞因子及肝纤维化指标的影响。方法 82例慢性乙型肝炎患者随机分为对照组（40例）和治疗组（42例），对照组采用常规保肝治疗，治疗组在对照组基础上加用氧化苦参碱治疗，疗程均为24周。治疗前后采用双抗体夹心（ELISA）法和RIA法测定血清转化生长因子β1（TGF-β1）、白细胞介素- 6（IL- 6）和透明质酸（HA）、Ⅳ型胶原（C-Ⅳ）、层粘连蛋白（LN）水平；20名健康人作正常对照组。结果 慢性乙型肝炎患者血清HA、C-Ⅳ、LN和TGF-β1、IL- 6水平较正常对照组显著升高（P＜0.01）。治疗组治疗24周后上述5项指标水平较治疗前及对照组均有显著降低（P＜0.01）。结论 氧化苦参碱能影响慢性乙型肝炎患者TGF-β1和IL- 6等细胞因子的水平，可能是其治疗肝纤维化的机制之一。

Effects of Oxymatrine on Serum Cytokines and Hepatic Fibrotic Indexes in Patients with Chronic Hepatitis B

OBJECTIVE: To study the effect of oxymatrine on serum cytokines and hepatic fibrotic indexes in patients with chronic hepatitis B (CHB). METHODS: Eighty-two CHB patients were randomly assigned to the control group treated with Western routine therapy and the treated group treated by Western routine therapy plus oxymatrine. The treatment course was 24 weeks. Another group with 20 healthy subjects was set as the normal control group. The serum levels of transforming growth factor-beta1 (TGF-beta1), interleukin-6 (IL-6), hyaline acid (HA), collagen type IV (C-IV) and laminin (LN) were measured by ELISA and RIA before and after treatment. RESULTS: The serum levels of TGF-beta1, IL-6, HA, C-IV and LN in CHB patients were significantly higher than those in the normal control group (P < 0.01). After 24-week treatment with oxymatrine, these abnormal indexes in the treated group were significantly lowered, as compared with those before treatment and in the control group, the differences were significant (all P < 0.01). CONCLUSION: Oxymatrine could lower the levels of cytokines, including TGF-beta1, IL-6, etc. in patients with CHB, which might be one of the mechanisms of its action in reversing liver fibrosis.