探讨服中求阳与阳中求阴对谷氨酸单钠大鼠下丘脑—垂体— …

目的：研究弓状核（ＡＲＣ）毁坏后下丘脑－垂体－肾上腺－胸腺（ＨＰＡＴ）轴的变化以及与肾阴肾阳的内在联系。方法：给 出生后（第２、４、６、８、１０天）大鼠每天皮下注射左旋谷氨酸单钠（ＭＳＧ）４ＭＧ／Ｇ损毁下丘脑ＡＲＣ，成年后分别以左 右归饮灌胃（每天５ｇ／ｋｇ）结果：ＭＳＧ大鼠下丘脑室旁核促肾上腺皮质激素释放激素（ＣＲＨ）阳笥细胞及垂体前叶肿肾上腺皮质激素（ＡＣＴＨ）分泌细胞数量明显多于生理盐水组，

Effect of Regulation of Kidney Yin and Kidney Yang on Hypothalamus Pituitary Adrenal Thymus Axis in Monosodium L Glutamate Rats

Objective: To study the interelationship between hypothalamus pituitary adrenal thymus (HPAT) axis and Kidney Yang and Kidney Yin in arcuate nucleus destroyed rats. Methods: Monosodium glutamate (MSG) 4 mg/g body weight was given subcutaneously to neonatal rats at 2, 4, 6, 8 and 10th day after born to destroy the ARC, and Zuogui pill or Yougui pill (5g/kg daily) was given respectively by gastrogavage when the model rats entered adulthood. Results: Immunohistochemical staining showed that in the model group the number of corticotropin releasing hormone (CRH) positive neurons in hypothalamic paraventricular nucleus and anterior pituitary adrenotrophin (ACTH) positive secretory cells, which stained deeply, were more than those in the control group, the adrenal fasciculate zone disturbed with increased cells and obviously dilated sinusoid. The thymus atrophied with lymphocyte proliferation apparently lower, blood corticosterone, ACTH content and hypothalamic CRF level higher in the model than those in the control. Zuogui pill could improve above mentioned pathophysiologic changes effectively but Yougui pill could not. Conclusion: Process of pathophysiololgy of HPA axis hyperfunction accompanied cell mediated immunodeficiency may belong to the category of Kidney Yin Deficiency Syndrome.