中药天癸方对雄激素致大孕大鼠血leptin及垂体促性腺激素的影响

目的：探讨中约天主对雄激素致不孕大鼠肥胖及生殖功能的影响。方法：运用放射免疫法测定天癸方治疗前后血ｌｅｐｔｉｎ，促卵泡激素，促黄体生成素的变化；同时对ＡＳＲ能量状态指标进行评估。结果：ＡＳＲ呈高代谢，能量失衡肥胖状态，其血ｌｅｐｔｉｎ水平明显守加。ｌｅｐｔｉｎ与体重呈正相关，与ＦＳＨ，ＬＨ呈负相关。

Effect of tiangui recipe on serum leptin and pituitary gonadotropin in androgen-sterilized rats]

OBJECTIVE: To explore the effect of Tiangui Recipe (TGR) on obesity and reproductive function in androgen-sterilized rats (ASR). METHODS: ASR model was established by subcutaneous injection of testosterone propionate to SD female rats of 9 days age. Change of serum leptin, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in model rats of 115 days old before and after feeding TGR was measured by radioimmunoassay (RIA). Meanwhile, criteria of energy state, including diurnal food intake, retroperitoneal fat depot weight and body weight, were measured and evaluated. RESULTS: ASR had the characteristics of high metabolic rate, energy imbalance and obesity. Serum level of leptin increased and FSH, LH decreased significantly (P < 0.01) in model rats as compared with the normal rats. Significant positive correlation between leptin level and body weight (r = 0.8977, P < 0.001) was observed, and negative correlation was seen between FSH, LH and leptin (r = -0.7517, P < 0.001; r = -0.8444, P < 0.001, respectively). The endocrinous changes of ASR all returned to normal range after the TGR treatment. CONCLUSION: Obesity and concerned hypogonadotropic hypogonadal anovulation were expressed in ASR. TGR may play an important role in reducing weight and inducing ovulation.