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丹参对肝癌转移复发防治作用的研究
引用本文:孙婧璟,周信达,刘银坤.丹参对肝癌转移复发防治作用的研究[J].中国中西医结合杂志,1999,19(5):292-295.
作者姓名:孙婧璟  周信达  刘银坤
作者单位:上海医科大学中山医院肝癌研究所,第二军医大学东方肝胆外科医院
基金项目:美国CMB基金,国家自然科学基金,国家“九五”攻关课题
摘    要:目的:观察丹参对SMMC-7721肝癌细胞株(7721细胞)侵袭粘附能力和对裸鼠人肝癌切除术后转移复发的影响。方法:以流式细胞仪测量丹参对7721细胞表面细胞间粘附分子-1(ICAM)-1)的表达的影响。经细胞侵袭实验观察央参地SMVC-7721细胞侵袭运动能力及对己与纤维连接蛋白(FN)粘附的7721细胞脱落的影响。以MTT法测量丹参对7721细胞与FN粘附的影响。以细胞粘附实验观察丹在对772

关 键 词:肝癌  肿瘤转移  粘附分  抗粘附  丹参  中医药疗法

Study on Preventive and Therapeutic Effect of Radix Salviae Miltiorrhizae on Recurrence and Metastasis of Liver Cancer
Authors:Sun Jingjing  Zhou Xinda  Liu Yinkun Liver Cancer Institute  Zhongshan Hospital  Shanghai Medical University  Shanghai
Abstract:Objective: To study the inhibitory effect of Radix Salviae Miltiorrhizae (RSM) on adhesive and invasive ability of SMMC 7721 liver cancer cells, and on the metastasis and recurrence on post hepatectomy liver cancer in nude mice. Methods: Effect of RSM on SMMC 7721 cell membrane intercellular adhesive molecule 1 (ICAM 1) expression was observed by immunofluorescence flow cytometry; effect on invasive ability and of SMMC 7721 cell and the detachment of which attached to fibronectin (FN) by cell migration experiment; effcet on adhesion of SMMC 7721 cell with FN by MTT method and effect on adhesion between 7721 7721, 7721 lymphocyte and 7721 endothelial cell by cell adhesion experiment. LCI D20 human liver cancer metastasis model, after hepatectomy, was used to observe the effect of RSM on recurrence and metastasis of liver carcinoma in nude mice. Results: ICAM 1 expression in SMMC 7721 cells incubated with RSM was significantly lower than that in cells did not treated with RSM. RSM could inhibit the invasive ability of SMMC 7721 cell and made the cells already attached to FN exfoliated. It could also inhibit the adhesion of 7721 7721, 7721 lymphocyte and 7721 endothelial cell. And RSM showed preventive and therapeutic effect on intrahepatic and remote metastasis/recurrence of early and late human post hepatectomy liver cacer in nude mice. Conclusion: RSM could inhibit the invasion and adhesion of SMMC 7721 cell and could also prevent and inhibit metastasis and recurrence of human liver cancer after hepatectomy in nude mice.
Keywords:liver cancer  tumor metastasis  adhesion molecule  anti  adhesion  Radix Salviae Miltiorrhizae  
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