健脑益智汤对类阿尔茨海默病大鼠学习记忆的影响和机制

目的　观察健脑益智汤对类阿尔茨海默病（AD）大鼠学习记忆的影响，并探讨其可能的作用机制。 方法　老年大鼠侧脑室注射聚集态的Aβ25 35建立复合式类AD大鼠模型，分为模型组、盐酸多奈哌齐组〔0.525 mg/（kg·d）〕、健脑益智汤小剂量组〔10.6 g/（kg·d）〕、健脑益智汤中剂量组〔21.2 g/（kg·d）〕和健脑益智汤大剂量组〔42.4 g/（kg·d）〕,治疗21天,并且采用Morris水迷宫测试各组大鼠学习记忆能力，并检测血清乙酰胆碱酯酶（AchE）、丁酰胆碱酯酶(BchE)。 结果 盐酸多奈哌齐组，健脑益智汤小剂量组、中剂量组和大剂量组逃避潜伏期均较模型组减少 (P<0.01,P<0.05)；而健脑益智汤小、中及大剂量组与盐酸多奈哌齐组比较,差异均无统计学意义（P>0.05）。健脑益智汤大、中剂量组对AchE、BchE活性虽有降低的趋势，但与盐酸多奈哌齐组比较，差异无统计学意义（P>0.05）；并且对类AD大鼠学习记忆能力的改善作用二者也无明显差别。结论 健脑益智汤可以通过对胆碱酯酶活性影响，进而改善类AD大鼠的学习记忆能力。

Effect and Mechanism of Jiannao Yizhi Decoction on Learning and Memory in Rats with Similar Alzheimer's Disease

Objective To observe the effect of Jiannao Yizhi Decoction （JNYZD） on learning and memory in rats with similar Alzheimer＇s disease （AD） , and to investigate its possible mechanism. Methods The composite AD rat model was established by injecting aggregated Aβ25-35 into the lateral cerebral ventricle of senile rats, and all the modeled rats were divided into 5 groups, the model group, the Donepezil group, the high-, middle-, and low-dose JNYZD group. All rats, except those in the model group, were treated respectively with Donepezil and JNYZD at the daily dose of 0. 525 mg/kg, 42.4 g/kg, 21.2 g/kg, 10. 6 g/kg for 21 days. The ability of learning and memory of rats in different groups was tested using Morris water maze, and the activity of acetylcholine esterase （AchE） and butyrocholin esterase （BchE） in serum were determined, too. Results The escape latent period was shorter in all medicated group than in the model group （P 〈0.01 or P 〈0. 05） , and it was insignificantly different among all medicated groups （P 〉0.05）. A decreasing trend of Ache and BchE activity presented in the high- and middle-dose JNYZD groups, but insignificant difference was shown as compared these indexes respectively with those in the Donepezil group. Furthermore, the improvement of learning and memory in similar AD rats was insignificantly different between the Donepezil group and the JNYZD groups （P 〉0. 05）. Conclusion JNYZD can improve the learning and memory ability of similar AD rats by influencing the activity of cholinesterase.