大蒜素对NIH3T3细胞增殖及胶原合成的影响

目的 研究大蒜素对NIH3T3细胞生长增殖和Ⅰ型胶原合成的影响，探讨其抗纤维化作用机理。方法 大蒜素加入NIH3T3细胞培养液，以丹参为对照药物，测定H^3-thymidineDNA掺入量；碱消化法检测细胞培养液中羟脯氨酸含量，荧光免疫染色方法检测NIH3T3细胞Ⅰ型胶原蛋白的表达一结果大蒜素在0．2～5μg／mL剂量范围内剂量依赖的抑制NIH3T3细胞生长和增殖；药物作用后，细胞培养液中的羟脯氨酸含量减少，Ⅰ型胶原蛋白的表达减少。结论 大蒜素对NIH3T3细胞生长增殖有抑制作用，减少细胞胶原的合成，减少Ⅰ型胶原的表达，可能具有抗纤维化作用。

Effects of Garlicin on NIH3T3 Cell Proliferation and Collagen Synthesis

OBJECTIVE: To investigate the effects of garlicin on fibroblasts proliferation and type I collagen synthesis and explore its anti-fibrosis mechanism. METHODS: Garlicin was added into the culture fluid of NIH3T3 cell, taking Radix Salviae miltiorrhizae as the control medicine. The spiking of H3-thymidine DNA was detected, also the hydroxyproline (HOP) concentration in the culture fluid by alkali digestion method and the protein expression of type I collagen in NIH3T3 cells by immunofluorescent staining. RESULTS: The NIH3T3 cell growth and proliferation rate were obviously reduced after garlicin treatment concentration-dependently in range of 0.2 - 5 microg/mL; HOP level and protein expression of type I collagen also lowered. CONCLUSION: Garlicin could inhibit NIH3T3 cell proliferation, reduce the synthesis and protein expression of type I collagen so as to exert the anti-fibrosis effect.