补肾药对雄激素致无排卵大鼠垂体及卵巢的形态学变化观察

SD雌性大鼠幼鼠,D 9日龄丙酸睾九酮处理。D 70日龄阴道涂片两个性周期。D 80日龄灌服补肾中药水溶液2周。用蒸馏水作为对照组。D 100日龄处死,取其垂体和卵巢,通过光镜、透射电镜观察形态学变化,并以亲和组化法检测卵巢内雌、孕激素受体。结果与对照组相比,灌服补肾中药后卵巢的间质腺增多,间质腺胞浆内脂滴减少;雌、孕激素受体增加,孕激素受体增加更明显。腺垂体细胞内"粒溶"、"自嗜"消失。提示补肾中药水溶液对大鼠卵巢、垂体的激素合成代谢有明显作用。并对卵巢的受体形成也有一定作用。

Morphological Changes of Pituitary Gland andOvary in Testosterone-induced Anovulatory Rat Treated with Chinese Medieinal Herbs of Replenishing Kidney

Injection of testosterone propionate 1.25mg were given to SD rats on 9 days of age, and fed with replenishing Kidney herbal decoction as well as with distilled water as control from 80 to 94 days of age. On 100 days of age, all the rats were sacrificed and the pituitary glands and ovaries were excised for examination. In the ovaries, increase in interstitial glands' count and lowering of lipid drop's quantity in cytoplasm of interstitial glandular cells were observed after the treatment, and intracellular "crinophagy" and "autophagy" disappeared in the pituitary gland. After treatment with replenishing Kidney drugs, ovarian estrogen receptors increased whereas the ovarian progesterone receptors raised even higher. It suggests that both ovaries and pituitary gland in testosterone induced anovulatory rats were affected by herbs of replenishing Kidney.