柴疏四君汤对肝郁、脾虚和肝郁脾虚模型大鼠性腺轴的影响

目的探查肝郁、脾虚、肝郁脾虚证模型大鼠性腺功能的变化和柴疏四君汤对其影响。方法大鼠按照随机数字表法分为正常对照、肝郁模型、肝郁给药、脾虚模型、脾虚给药、肝郁脾虚模型、肝郁脾虚给药组，共7组，每组10只。肝郁模型、脾虚模型、肝郁脾虚模型组及各相应给药组大鼠分别采用慢性束缚、饮食失节＋过度疲劳、慢性束缚＋饮食失节＋过度疲劳的方法，连续造模4周。于造模第15天，各给药组分别予柴疏四君汤3.57 g/（kg·d）灌胃，各模型组和正常对照组给予等量蒸馏水，连续14天。检测各组大鼠血中的促性腺激素释放激素（GnRH）、促卵泡刺激素（FSH）、促黄体生成素（LH）、雌二醇（E2）、睾酮（T）。结果与正常对照组比较，肝郁、脾虚及肝郁脾虚模型组GnRH、T、E2、FSH差异均有统计学意义（P〈0.05， P〈0.01），肝郁模型组LH升高（P〈0.05），肝郁脾虚模型组LH降低（P〈0.01）。与肝郁模型组比较，脾虚模型组T、FSH、LH降低，E2升高（P〈0.05），肝郁脾虚模型组FSH和LH亦降低（P〈0.05）；与脾虚模型组比较，肝郁脾虚模型组T升高（P〈0.05），FSH降低（P〈0.05）。分别与相应模型组比较，肝郁给药组FSH降低（P〈0.01）和LH升高（P〈0.05），脾虚给药组T升高，E2和LH 降低（P〈0.05， P〈0.01），肝郁脾虚给药组T降低（P〈0.01），GnRH、E2、FSH、LH升高（P〈0.05， P〈0.01）。结论中医肝郁、脾虚、肝郁脾虚3证模型均存在不同程度的性腺轴功能异常。柴疏四君汤对3证均有不同程度的调节作用，其中对肝郁脾虚证的性腺轴功能具有较为全面的改善作用。该结果为中医疏肝健脾方与肝郁脾虚证具有较高的关联性提供了一定的实验证据。

Effect of Chaishu Sijun Decoction on the Gonad Axis of Gan-qi Stagnation,Pi Deficiency,and C-,an-qi Stagnation Pi Deficiency Model Rats

Objective To observe changes of gonad functions of Gan-qi stagnation （GS）, Pi de- ficiency （PD）, Gan-qi stagnation Pi deficiency （GSPD） model rats, and the effect of Chaishu Sijun Decoction （CSD） on them. Methods Rats were randomly divided into 7 groups according to romdom digit table, i.e., the normal control group, the GS model group, the GS medication group, the PD model group, the PD medication group, the GSPD model group,and the GSPD medication group,10 in each group. Rats in the GS model group, the PD model group, and the GSPD model group were treated with chronic restraint, improper diet ＋ excessive fatigue, chronic restraint ＋ improper diet ＋ excessive fatigue. The model was established for 4 successive weeks. Starting from the 15th day of modeling, CSD at the daily dose of 3.57 g/kg was given by gastrogavage to them for 14 successive days. Equal volume of distilled water was given by gastrogavage to rats in each model group and the normal control group for 14 successive days. The blood contents of gonadotrophin releasing hormone （GnRH）, follicular stimulating hormone （FSH）, luteinizing hormone （LH）, estradiol （E2）, and testosterone （T） were detected in rats of each group. Resuits Compared with the normal control group, there was statistical difference in GnRH, T, E2, and FSH in the GS, PD, and GSPD model groups （P 〈0.05, P 〈0.01）. The content of LH was elevated in the GS model group （P 〈0.05） and declined in the GSPD model group （P 〈0.01 7. Compared with the GS model group, the contents of FSH, LH, and T decreased and E2 increased in the PD model group （all P 〈0.05） ; the contents of FSH and LH also declined in the GSPD model group （P 〈0.05）. Compared with the PD model group, the T content increased and FSH decreased in the GSPD model group （all P 〈0.05）. Compared with each corresponding model group, the FSH content decreased （P 〈0.01 ） and LH increased in the GS medication group; the T content increased, E2 and LH decreased （P 〈0.05, P 〈 0.01） in the PD medication group; the T content decreased （P 〈0.01 ）, GnRH, E2, FSH, and LH in- creased （P 〈0.05, P 〈0.01 ） in the GSPD medication group. Conclusions There exist different degrees of abnormal function of the gonad axis in the GS, PD, and GSPD models. CSD had certain regulatory effect on the 3 syndromes. Of them, it showed a more comprehensive role in improving the gonad function axis. Results of this experiment had provided the experimental evidence for higher correlation between CSD and GSPD syndrome.