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酒精性肝纤维化大鼠肝星状细胞及肝细胞凋亡和清肝活血方调控机制研究
引用本文:季光,王磊,柳涛,郑培永,邢练军.酒精性肝纤维化大鼠肝星状细胞及肝细胞凋亡和清肝活血方调控机制研究[J].中国中西医结合杂志,2007,27(5):421-425.
作者姓名:季光  王磊  柳涛  郑培永  邢练军
作者单位:上海中医药大学附属龙华医院脂肪肝研究室,上海,200032
基金项目:国家自然科学基金;上海市科技启明星跟踪培养计划
摘    要:目的 观察酒精性肝纤维化大鼠肝星状细胞(HSC)、肝细胞凋亡及清肝活血方对不同细胞凋亡的影响。方法 将大鼠分为空白组,模型组,清肝活血低、中、高剂量组,易善复组,清肝活血方低剂量组4.75g/(kg·d)]、中剂量组14.25g/(kg·d)]、高剂量组28.5g/(kg·d)]及易善复组66.5mg/(kg·d)],分别予相应药物灌胃,空白组和模型组以等量生理盐水代替,给药2周。比色法检测血清ALT、AST、γ-GT,HE、Masson染色观察肝脏炎症和纤维化程度。TUNEL法检测肝细胞凋亡,TUNEL-α-SMA双标记法检测HSC凋亡。结果 清肝活血方能降低大鼠血清ALT、AST、γ-GT水平,减轻炎症及纤维化程度(P〈0.05);诱导活化的HSC凋亡,减少肝细胞凋亡(P〈0.05)。结论 清肝活血方能有效减轻大鼠肝纤维化程度,并减少酒精引起的肝细胞凋亡,诱导活化的HSC凋亡。

关 键 词:清肝活血方  酒精性肝纤维化  肝星状细胞  肝细胞  凋亡
收稿时间:2006-08-04
修稿时间:2006-09-28

Apoptosis of Hepatic Stellate Cell and Hepatocyte and Regulatory Effects of Qinggan Huoxue Recipe on It in Alcoholic Liver Fibrosis Rat
Authors:JI Guang  WANG Lei  LIU Tao
Abstract:OBJECTIVE: To observe the apoptosis of hepatic stellate cell (HSC) and hepatocyte and the effect of Qinggan Huoxue Recipe (QHR) on it in alcoholic liver fibrosis (ALF) rats. METHODS: Rats were divided into six groups: the normal group, the model group, the QHR low dose (4.75 g/(kg x d)], medium dose (14.25 g/(kg x d)] and high dose (28.5 g/(kg x d)) groups, and the Essentiale (66.5 mg/(kg x d)] group. They were treated with respective drugs through gavage for 2 weeks, while the normal and model groups were given normal saline instead. The serum levels of ALT, AST and gamma-GT were measured by chromatometry, the degree of inflammation and fibrosis was observed by HE staining and Masson staining, and the apoptosis of hepatocyte and HSC were detected by TUNEL and TUNEL-alpha-SMA double immunolabeling respectively. RESULTS: Compared with those before treatment, the serum levels of ALT, AST and gamma-GT obviously decreased (P < 0.05), inflammation and fibrosis relieved (P < 0.05), and the apoptosis of hepatocyte reduced (P < 0.05), while that of activated HSC increased in the QHR groups after treatment (P <0.05). CONCLUSION: QHR can effectively relieve hepatic fibrosis, decrease hepatocyte apoptosis caused by alcohol and induce activated HSC apoptosis in ALF rats.
Keywords:Qinggan Huoxue Recipe  alcoholic liver fibrosis  hepatic stellate cell  hepatocyte  apoptosis
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