白花蛇舌草的免疫学调节活性和抗肿瘤活性

目的：研究白花蛇舌草的免疫学调节活性和抗肿瘤活性。方法：用^3H介入法分析了白花蛇舌草提取物（Oldenlandia diffusa extract,ODE）对小鼠脾细胞的增殖活性；用^51Cr游离实验测定了小鼠和人杀伤细胞对肿瘤细胞的特异性杀伤活性，用ELISA法和生物法研究了B细胞抗体的以及单核细胞的细胞因子产生，并用^3H介入法分析了单核细胞对肿瘤细胞的吞噬功能；用层析法、蛋白酶消化法和糖分解处理（即NaIO4处理）分析了白花舌草的化学成分。结果：ODE对小鼠脾细胞的增殖活性有很强的促进作用；ODE增强小鼠和人杀伤细胞对肿瘤细胞的特异性杀伤活性，增强B细胞抗体的产生以及单核细胞的细胞因子产生，并增强单核细胞对肿瘤细胞的吞噬功能，白花蛇舌草的化学成分为90道尔顿的糖蛋白。结论：ODE对小鼠的人有免疫调节作用，并通过刺激机体的免疫系统杀伤或蚕噬肿瘤细胞，这些活性可应用于临床调节免疫功能和治疗肿瘤等疾病。

Immunomodulatory Activity and Anti-Tumor Activity of Oldenlandia Diffusa in vitro

OBJECTIVE: To study the effect of Oldenlandia diffusa (OD) on lymphocytes and tumor cells in vitro. METHODS: Effects of OD extract (ODE) on proliferation of spleen cells of mice and the phagocytosis of monocytes to tumor cells using 3H incorporation were analysed, and effect on specific activity of natural killer (NK) cells to human and mice's tumor cells was determined using free 51 Cr experiments, effects on B cells' antibody production and cytokine of monocyte production were investigated by ELISA and biological method, the chemical composition of OD was analyzed by chromatography, protease digestion and sugar decomposition (NaIO4) test. RESULTS: ODE could vigorously promote the proliferative activity of spleen cells in mice, specific lethal activity of human and mice's NK cell to tumor cells, B cells' antibody production, monocytes' cytokine production and its phagocytosis to remove the tumor cells. By means of chromatography, protease E digestion and NaIO4 treatment, the result of chemical composition analysis showed that the component of OD was 90 kDa glycoprotein. CONCLUSION: ODE has immuno-modulating activity and anti-tumor activity in vitro through stimulating the immune system to kill or engulf tumor cells, which could be used clinically for immune function modulation and to treat tumor and other diseases.