黄芪对单疱病毒性角膜炎患者Th1/Th2细胞因子的调节作用

目的：探讨黄芪对单疱病毒性角膜炎(haerpes simplex keratits，HSK)患者血清Th1／Th2细胞因子水平的影响。方法：选择106例HSK患者随机分为黄芪治疗组和病毒唑治疗组，治疗前后分别采用双抗体夹心法酶联免疫吸附实验(ELISA)检测外周血白细胞介素-2(IL-2)，白细胞介素-4(IL-4)，白细胞介素-10(IL-10)，γ-干扰素(γ-IFN)水平；并以同样方法检测62名体检正常献血员作健康对照组。结果：HSK患者血清中IL-4、IL-10水平较健康对照组显著增高(P<0．01)；IL-2；γ-IFN水平较健康对照组显著降低(P<0．01)。黄芪治疗组患者治疗后，血清IL-4、IL—10水平显著降低(P<0．01)，而IL-2、γ-IFN水平显著增加(P<0．01)。病毒唑治疗组治疗前后上述指标无明显改变(P>0．05)。结论：黄芪能调节HSK患者体内Th1／Th2细胞因子的失衡状态，改善患者机体免疫功能紊乱状况，这对HSK患者治疗有重要意义。

Modulatory Effect of Astragalus Membranaceus on Th1/Th2 Cytokine in Patients with Herpes Simplex Keratitis MAO

OBJECTIVE: To explore the influence of Astragalus membranaceus (AM) on serum cytokines, Th1, including interleukin-2 (IL-2) and gamma-interferon (gamma-IFN), and Th2, including interleukin-4 (IL-4) and interleukin-10 (IL-10), in patients with herpes simplex keratitis (HSK). METHODS: One hundred and six HSK patients were randomly divided into the AM treated group and the ribavirin treated group. Levels of serum IL-2, IL-4, IL-10 and gamma-IFN of all the patients and 62 healthy person, selected from donors for control group, were determined by sandwich enzyme-linked immunosorbent assay (ELISA) technique. RESULTS: Levels of serum IL-4 and IL-10 in HSK patients were significantly higher and those of IL-2 and gamma-IFN were significantly lower than those in the healthy control (all P < 0.01). These parameters were significantly improved in the patients of the AM group after treatment, but with no change in patients of the ribavirin group. CONCLUSION: AM can modulate the imbalance state of Th1/Th2 in HSK patients, improve their immune function disturbance, that shows important significance in treating HSK.