天麻诱导骨髓间质干细胞分化为神经元样细胞的实验研究 |
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引用本文: | 董晓先,刘金保,董燕湘,肖庆忠.天麻诱导骨髓间质干细胞分化为神经元样细胞的实验研究[J].中国中西医结合杂志,2004,24(1):51-54. |
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作者姓名: | 董晓先 刘金保 董燕湘 肖庆忠 |
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作者单位: | 广州医学院病理生理学教研室,广州,510182 |
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基金项目: | 国家高技术研究发展计划(863计划),广东省广州市科技攻关项目 |
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摘 要: | 目的:探讨天麻对体外定向诱导大鼠骨髓间质干细胞(MSC)分化为神经元样细胞的影响:方法:通过贴壁法分离大鼠MSC,体外扩增培养。中药天麻定向诱导分化为类神经元样细胞光镜下观察细胞形态,免疫细胞化学法检测神经细胞特异性抗原标志物神经原烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)、巢蛋白(Nestin)。结果:大鼠MSC可通过贴壁法成功分离并可在体外大量扩增。天麻诱导2h后大部分可分化为神经元样细胞,出现胞体和突起,免疫细胞化学染色NSE、Nestin呈阳性,GFAP阴性。结论:大鼠MSC可诱导分化为神经元样细胞。
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关 键 词: | 天麻 诱导 骨髓间质干细胞 神经元样细胞 实验研究 中药 |
修稿时间: | 2003年1月20日 |
Experimental Study on Effect of Gastrodia in Inducing the Differentiation of Mesenchymal Stem Cells into Neuron-like Cells |
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Authors: | DONG Xiao-xian LIU Jin-bao DONG Yan-xiang |
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Abstract: | OBJECTIVE: To investigate the effect of gastrodia in extracorporeal oriented inducing the differentiation of mesenchymal stem cells into neuron-like cells. METHODS: Mesenchymal stem cells were separated from bone marrow of rats by wall sticking method, amplifying cultured in vitro, and differentiated into neuron-like cells by oriented induction with gastrodia. The morphology of cells was observed under light microscopy, neuro-specific enolase (NSE), nestin and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry. RESULTS: Rats mesenchymal stem cells could be separated and amplified in vitro. After being induced by gastrodia for 2 hrs, most of the cells would be differentiated into meuron-like cells, revealing cytodendrite. By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP. CONCLUSION: Rats' mesenchymal stem cells could be induced to differentiate into neuron-like cells. |
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Keywords: | gastrodia bone marrow mesenchymal stem cell nerve cell oriented differentiation |
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