中西药复合配方999感冒灵的药理学实验研究

目的 研究中西药复合配方999感冒灵的药物协同性、互补性的药理作用特点及其中西药配比的理论基础。方法 采用冰醋酸引起小鼠扭体反应、大鼠角叉菜胶引起的足跖肿胀、小鼠迟发型超敏反应、内毒素引起大鼠和兔发热现象，对999感冒灵的镇痛、解热、抗炎及迟发型超敏反应作用进行研究；对999感冒灵的中西药部分进行对比研究，并分别对999感冒灵中药部分及西药部分拮抗对乙酰氨基酚所致的小鼠肝损伤的作用进行研究；RT-PCR法分析999感冒灵对相关细胞因子表达的影响。结果 999感冒灵具有明显的解热镇痛作用；对角叉菜胶所致炎症有明显拮抗作用；对内毒素所致发热有一定的拮抗作用；对于感染肺炎双球菌的小鼠脾组织细胞因子表达量有一定促进作用；其中药部分对对乙酰氨基酚所致小鼠肝损伤有一定保护作用。结论 999感冒灵具有较强的解热镇痛抗炎作用，中西药的协同性、互补性明显；其中药成分具有保肝作用。

Experimental Study on the Pharmacology of 999 Ganmaoling,a Compound Recipe of Chinese and Western Materia Medica

OBJECTIVE: To study the pharmacologic characteristics in synergism and complementation of 999 Ganmaoling (GML), a compound recipe composed of Chinese and Western materia medica (CMM & WMM), as well as its theoretical basis of matching of Chinese and Western materia medica. METHODS: The torsion response induced by glacial acetic acid in mice, toe swelling induced by carrageenanin rats, delayed hypersensitive response in mice and fever induced by endotoxin in rats and rabbits were used to comparatively study the actions of CMM & WMM in GML. The effect of CMM in antagonizing liver damage caused by WD (acetaminophen) in mice was also studied. RT-PCR method was used to analyze the expression of related cytokines. RESULTS: GML showed a significant antipyretic and analgesic effect, it could inhibit the carrageenan induced inflammation, antagonize the endotoxin induced fever, and promote the amount for expression of cytokines in rats' splenic tissue with pneumococci infection to some extent. The CMM in GML showed certain protective effect on acetaminophen induced liver damage. CONCLUSION: GML has a potent antipyretic, analgesic and anti-inflammatory effects, CMM & WMM in GML showed markedly synergism and complementation, and CMM in it has liver protective effect.