氧应激对豚鼠心肌细胞线粒体膜电位的影响及益心康胶囊含药血清保护作用的研究

目的观察氧应激对豚鼠心肌细胞线粒体的影响，探讨抗心肌缺血中药复方——益心康胶囊(H303)含药血清对氧应激损伤的保护作用机制。方法利用激光共聚焦显微镜，观察羟自由基损伤体系(Fe2＋／H2O2)与豚鼠心肌细胞共同孵育30～60min后对心肌细胞线粒体膜电位的影响。结果豚鼠心肌细胞氧应激后，可致线粒体细胞色素氧化酶活性降低，以及荧光染料Rh123所显示的线粒体膜电位明显降低。H303含药血清可明显减轻上述损伤，表现为明显增强豚鼠心肌细胞线粒体氧化酶活性，使心肌细胞存活率增加；并具有保护心肌细胞膜电位的作用。结论氧应激可造成心肌线粒体膜电位降低。益心康胶囊含药血清可保护线粒体膜电位，从而发挥其膜稳定作用。

Protective Effect of Yixinkang Capsule Containing Serum on Oxygen Stress Damaged Mitochondrial Membrane Potential in Guinea pig Myocytes

Objective: To explore the protective effect and mechanism of Yixinkang (H303) capsule, a composite preparation of Chinese herbal medicine for antagonizing myocardial ischemia, containing serum on oxygen stress damaged mitochondrial membrane potential (MMP) in guinea pig myocytes. Methods: Myocyte damage was induced by incubating myocytes with hydroxy free radical (Fe 2 /H 2O 2) generating system for 30-60 mins, then H303 containing serum was added in to observe the protective effect. MMP was detected by laser scanning confocal microscopy. Results: After oxygen stress, the cytochrome oxidase (CCO) activity and MMP in mitochondria significantly decreased. These changes could be significantly lessened by H303 containing serum, demonstrated as obviously increase of CCO activity and myocyte survival rate. H303 containing serum also showed a significant protective effect on membrane potential of myocytes. Conclusion: Oxygen stress could cause decrease of MMP in myocytes and H303 containing serum could protect it to maintain the membrane stability of mitochondria.