比较黄芪和活血方对兔脑再灌注损伤血栓素A2及前列环素和环核苷酸的影响

兔脑缺血３０ｍｉｎ再灌注４５ｍｉｎ后，脑内血栓素Ｂ_２（ＴＸＢ_２）升高和６－酮－前列腺素Ｆ_（１α）（６－酮－ＰＧＦ_（１α））含量降低（Ｐ＜０．０５），血浆和脑环磷酸腺苷（ｃＡＭＰ）含量升高（Ｐ＜０．０１和Ｐ＜０．０５）。于缺血前分别静脉注射黄芪（３．３ｇ／ｋｇ）、活血方（２．５ｇ／ｋｇ）及益气活血方（４．４ｇ／ｋｇ），发现活血方和益气活血方均可抑制再灌注后脑ＴＸＢ_２的升高，提高脑６－酮－ＰＧＦ_（１ａ）含量（Ｐ＜０．０５）。活血方还可抑制脑ｃＡＭＰ的升高（Ｐ＜０．０５），黄芪则对此无明显影响。提示益气活血法通过改善脑局部ＴＸＡ２／ＰＧＩ_２平衡而起作用，主要为活血药物作用。

Comparison of Effect of Astragalus membranaceus and Huoxuefang on Thromboxane, Prostacyclin and Adenosine Cyclic Monophosphate in Cerebral Reperfusion Injury in Rabbits

The cerebral ischemia rabbit model was made by using the occlusion of four vessels. The results showed that TXB_2 and cAMP contents in brain tissues and the latter in plasma markedly increased (P<0. 05, P<0 . 01), the 6-keto-PGF_la in brain tissues significantly lowered (P<0 . 05) in ischemia formed 30 minutes and 45 minutes after reperfusion. After intravenous injection of Astragalus membranaceus (AM) extracts (3.3g/kg), Huoxuefang (HXF) and Yiqi Huoxue Fang (YQHXF)consisted of AM and HXF before ischemia,the marked increase of TXB_2 contents after reperfusion was inhibited (P<0. 05) and the 6-keto-PGF_la in brain tissues after reperfusion were increased (P<0. 01) in HXF and YQHXF group, which change the AM extracts didn't have (P<0. 05). HXF could markedly inhibit the increase of cAMP in brain tissues after reperfusion (P<0.05),while the AM extracts and YQHXF couldn't (P>0 . 05).All above-mentioned suggested that the balancedisorder of TXA_2/PGI_2 in brain tissues might participate in the occurrence of cerebral reperfusion injury and YQHXF might act against this injury by means of improving the balance of TXA-2/PGI-2 in brain tissues, which was mainly released by HX drugs of it.