雷公藤多甙对大鼠胶原免疫性关节炎及佐剂性关节炎黏膜免疫功能影响的对比研究

目的观察雷公藤多甙对大鼠胶原免疫性关节炎（collagen-Ⅱ induced arthritis，CIA）及佐剂性关节炎（adjuvant arthritis，AA）黏膜免疫功能的影响。方法分别以Ⅱ型胶原加完全弗氏佐剂和完全弗氏佐剂单独致炎免疫大鼠造成CIA和AA模型，以雷公藤多甙治疗，观察大鼠黏膜、全身免疫指标（外周血T细胞亚群）以及关节局部炎症因子（IL-6、TNF—α、COX-2和NF-κB等）的变化。结果CIA模型组派伊尔结（Peyer’s patch，PP）CD4^＋、血中CD4^＋、CD8^＋均升高；AA模型组PPCD4^＋降低，CD8^＋升高，血中的CD4^＋，CD8^＋均升高。雷公藤对两模型组的PP和血中T细胞亚群存在不同影响。两种模型大鼠关节可见高水平IL-6、TNF—α、COX-2和NF—κB表达，雷公藤多甙可以抑制这些炎症介质的表达。结论两种关节炎模型在黏膜免疫反应方面及雷公藤多甙对这两种模型的影响存在异同。

Comparative Study on Effect of Tripterygium Polyglycosides on Mucous Immune Function of Rat Models of Arthritis Induced by Collagen Ⅱ and by Adjuvant

OBJECTIVE: To observe the effects of Tripterygium polyglycosides (TWP) on mucous immune function in rat models of arthritis induced respectively with collagen-II induced arthritis (CIA) & adjuvant arthritis (AA). METHODS: CIA and AA model rats were induced by immunization with collagen II emulsified with complete Freund's adjuvant and complete Freund's adjuvant respectively and treated with TWP. Rats' mucus, systemic immunological indexes (peripheral subsets of T cells), local inflammatory factors (IL-6, TNF-alpha, COX-2,and NF-kappaB, etc. ) were observed. RESULTS: In CIA model group, CD4+ in Peyer's Patch (PP), peripheral CD4+ and CD8+ positive T cells all raised, while in the AA model group, CD4+ lowered and CD8+ raised on PP, with both subsets increased. Effects of TWP on T lymphocyte subsets in PP and blood of the two models were different. High leveled IL-6, TNF-alpha, COX-2 and NF-kappaB expression could be seen in both model groups, and these inflammatory media could be inhibited by TWP. CONCLUSION: There exist similarities and differences between the two models in aspects of mucus immune response and effect of TWP on them.