丹参注射液协同地塞米松抑制支气管哮喘大鼠肺内IL-13和嗜酸性粒细胞趋化因子的表达

目的 探讨丹参注射液协同地塞米松抑制支气管哮喘气道变应性炎症作用的分子机制。方法 40只SD大鼠随机分成正常组、模型组、地塞米松组、丹参组和联合组，每组8只。除正常组不造模外，其余4组均造成哮喘模型，且各组给予相应的药物。HE染色行肺组织病理学检查；收集支气管肺泡灌洗液（BALF），行白细胞（WBC）及嗜酸性粒细胞（Eos）计数；应用免疫组织化学SP法和逆转录-聚合酶链反应（RT—PCR）测定肺组织白细胞介素-13（IL-13）和嗜酸性粒细胞趋化因子（Eotaxin）表达。结果 病理组织学显示：丹参组大鼠呈中度炎症变化，地塞米松组、联合组呈轻度炎症变化。BALF中WBC及Eos计数：3个用药组较模型组明显减少（P〈0．05），联合组减少的程度大于地塞米松组、丹参组（P〈0．05或P〈0．01）。肺组织IL-13和Eotaxin表达：3个用药组较模型组明显减少（P〈0．05），且联合组减少程度大于地塞米松组、丹参组（P〈0．05或P〈0．01）；IL-13和Eotaxin表达呈正相关（r=0．92，P〈0．01）。结论 丹参注射液可降低哮喘模型鼠肺内IL-13和Eotaxin的表达，与地塞米松有协同发挥抗气道炎症作用。

Inhibitory Effects of Salvia Miltiorrhiza Injection Coordinated with Dexamethasone on Interleukin-13 and Eotaxin Expression in Lung of Asthmatic Rats

ObjectiveTo investigate the molecular mechanism of inhibitory effect of Salvia miltiorrhiza Injection (SMI) coordinated with dexamethasone (DXM) on allergic airway inflammation in asthmatic rats. Methods Forty SD rats were randomly divided into 5 groups equally: the normal group, the asthma model group, the DXM group, the SMI group and the DXM SMI group, they were treated with correspondant herbal medicines. Pathologic changes of lung tissue were obseved with HE stain, count of WBC and eosinophil (Eos) in bronchoalveolar lavage fluid (BALF) were estimated and the expressions of interleukin-13 (IL-13) and Eotaxin in lung tissue were measured by RT-PCR and SP method of immunohistochemistry assay. Results There was moderate inflammation in lung tissue in the SMI group, and mild inflammation in the DXM SMI and the DXM group, which was similar to that in the normal group. Compared with the asthma model group, Eos and WBC count in BALF and the expression of IL-13 and Eotaxin in the lung tissue were significantly lower in the three treated groups (P<0.05), particularly in the DXM SMI group, showing a significant difference as compared with the other two groups (P<0.05 or P<0.01). Additionally, IL-13 expression was positively correlated with Eotaxin expression (r=0.92, P<0.01). Conclusion SMI could inhibit the expression of IL-13 and Eotaxin in the lung of asthmatic rats, showing inhibitory effects synergistic with DXM on airway inflammation.