恒古骨伤愈合剂促进兔坏死股骨头内核心结合因子α1基因表达的实验研究

目的 研究中药恒古骨伤愈合剂（简称中药合剂）对兔坏死股骨头内核心结合因子α1（cbfα1）基因表达的影响。方法 用大肠杆菌内毒素（LPS，10μg／kg体重）间隔24h两次静脉注射加甲基泼尼松3次肌肉注射制作的兔股骨头坏死模型，用中药合剂治疗后取股骨头行免疫组化，并提取总RNA行实时荧光定量多聚酶链反应（PCR），观察兔股骨头内cbfα1基因表达的动态变化特点。结果 给药第4周时，中药组与模型组股骨头内cbfα1基因表达均为阴性，至第8、12周时，中药组免疫组化结果为弱阳性，模型组为阴性；至第12周时中药组股骨头内cbfα1mRNA的表达量是模型组的2．87倍。正常给药组与空白组比较差异无显著性。结论 中药恒古骨伤愈合剂可促进坏死α股骨头内源性cbfα1基因的表达，以用药时间长者效果明显。

Experimental Study on Osteoking in Promoting Gene Expression of Core Binding Factor alpha 1 in Necrotic Femoral Head of Rabbits

OBJECTIVE: To study the effects of Osteoking in promoting gene expression of core binding factor alpha 1 (cbfalpha 1) in necrotic femoral head of rabbits. METHODS: Rabbit model of femoral head necrosis (FHN) was induced by intravenous injection of lipopolysaccharide (LPS) 10 microg/kg body weight twice with an interval of 24 h and intramuscular injection with methyl prednisone (MPS) 20 mg/kg body weight 3 times. The dynamic changes of cbfalpha 1 gene expression in the femoral head were observed with immunohistochemistry and real-time quantitative PCR. RESULTS: The protein expression of cbfa 1 gene was negative in both model and treatment groups at the 4th week, it turned to weakly positive in the treatment group at the 8th and 12th week but still negative in the model group. The mRNA expression of cbfalpha 1 in the treatment group was 2.87 times that in the model group at the 12th week. There was no significant difference in cbfalpha 1 expression between the normal rabbits with or without Osteoking treatment. CONCLUSION: Osteoking could promote the endogenous cbfalpha 1 expression in the FHN, the effect is better along with the prolonging of the time applied. But it showed no affection on cbfalpha 1 expression in the normal femoral head of rabbits.