黄芪注射液对大鼠腹膜细胞凋亡与增殖平衡的作用

目的:探讨中药黄芪注射液对腹膜细胞凋亡和增殖细胞核抗原(PCNA)表达的影响。方法:在正常 SD 大鼠上建立腹膜透析模型,用含黄芪注射液(200 mg/d,400 mg/d)的生理盐水灌注腹腔,20 ml/次,每日2次;用生理盐水作对照。4周后杀检,取腹膜用 LSAB 试剂盒和 TUNEL 试剂盒检测腹膜细胞凋亡和PCNA 表达。结果:黄芪能显著诱导腹膜间皮细胞凋亡,可刺激 PCNA 表达增加,PCNA 表达量呈浓度依赖性。结论:黄芪注射液加入腹透液中可调节腹膜细胞消长平衡。

Effect of Astragalus Injection on Balance between Peritoneal Cell Apoptosis and Proliferation in Dialysis Rats Model

To study the effect of Astragalus Injection(AI)on peritoneal cells apoptosis and proliferative cells nuclear antigen(PC- NA)expression.Methods:The animal model of peritoneal dialysis was established in normal SD rats.AI (20 ml)in different concentra- tions was perfused to the modal,twice a day with daily dose of 200 mg or 400 mg.By the end of the 4th week,the peritoneum was tak- en out,the levels of peritoneal cell apoptosis and PCNA expression were detected immunohistochemically with LSAB kit and TUNEL kit,and compared with those in the control treated with normal saline.Results:Al could significantly induce the apoptosis of peritoneal mesothelial cells and increase the PCNA expression concentration-dependently.Conclusion:AI could regulate the balance between the peritoneal cells apoptosis and proliferation of peritoneal mesothelial cells when added in the peritoneal dialysate.