补肾清利活血法对大鼠残肾细胞外基质影响的实验研究

目的：探讨补肾清利活血法延缓肾小球硬化的有效性和作用环节。方法：在５／６肾切除残肾模型大鼠的基础上，运用免疫组织化学及常规病理等方法观察补肾清利活血法对肾小球细胞外基质（ＥＣＭ）蓄积及其部分调控机制的影响。结果：残肾模型大鼠术后１周开始给予补肾清利活血中药，术后５周血肌酐（ＳＣｒ）、尿素氮（ＢＵＮ）、尿蛋白显著降低；病理结果示ＥＣＭ多种成分（Ⅰ和Ⅳ型胶原、层粘连蛋白）的蓄积显著减少，肾小球内细胞数

Experimemtal Study on Effect of Bushen Qingli Huoxue Treatment on Glomerular Extracellular Matrix of Remnant Kidney in Rats

OBJECTIVE: To study the effect of Bushen Qingli Huoxue (BQH) treatment on glomerular extracellular matrix (ECM) and in retarding glomerular sclerosis in rats and to explore the mechanism and key of regulation. METHODS: Animal model was established by 5/6 nephrectomy and the study was carried out by using immune histochemical and pathological methods. Treatment of BQH started 1 week after operation. RESULTS: After being treated with BQH, the serum creatinine (SCr), blood urea nitrogen (BUN) and urinary protein excretion reduced markedly. Pathological examination showed accumulation of ingredients of glomerular ECM such as type I, IV collagen and laminin decreased significantly, the number of glomerular cells and proliferation cell nuclear antigen positive cells also decreased obviously, and showed that the glomerular sclerosis was retarded. The effect of BQH was similar to that of Enapril, but superior to that of Enapril in reducing BUN and SCr. CONCLUSION: BQH has the effects of reducing ECM accumulation, improving renal function and retarding glomerular sclerosis.