清热利湿方防治兔胆色素结石形成的实验研究

目的动态观察清热利湿方对兔胆色素结石模型胆汁成分的影响。方法取健康家兔40只，随机分为对照组，细菌感染组，清热利湿中药大、小剂量组；造成胆色素结石模型，观察胆汁中总胆红素（TB）、游离胆红素（UCB）、总胆汁酸（TBA）、钙离子（Ca^2＋）、细菌性和内源性β-葡萄糖醛酸酶（B-Gase）活性的变化。结果清热利湿中药能明显降低胆汁中TB、UCB、Ca^2＋含量（P〈0．05），增加胆汁中总胆汁酸含量（P〈0．05），并能降低胆汁中细菌性和内源性β—Gase活性（P〈0．05）。结论清热利湿方具有很好的降低胆汁成石性的作用。

Effect of Clearing Heat and Removing Dampness Method on Formation of Pigment Gallstones in Rabbits

OBJECTIVE: To observe dynamically the effect of drugs for clearing heat and removing dampness (CHRD) on biliary components in rabbits with pigment gallstones (PGS). METHODS: Forty rabbits were established into PGS model and randomly divided into 3 groups, the bacterial infection group, the CHRD low-dose group and the CHRD high-dose group. Besides, a normal group was set up with healthy rabbits for control. Changes of total bilirubin (TB), unconjugated bilirubin (UCB), total bile acid (TBA), Ca2+, bacterial and endogenous beta-glucuronidase (beta-Gase) in bile were observed. RESULTS: CHRD drugs significantly decreased the contents of UCB, Ca2+, bacterial and endogenous beta-Gase (P < 0.05), and increased TBA in bile (P < 0.05). CONCLUSION: CHRD drugs have good effect in reducing the lithogenesis of the pigment gallstones.