小檗胺对多药耐药K562/Adr细胞作用的研究

目的研究小檗胺诱导人白血病K562／Adr细胞凋亡及逆转多药耐药的作用及机理。方法采用MTT法测IC50值，流式细胞仪Annexin V FITC-PI法检测细胞凋亡发生率，PI染色法检测凋亡峰及细胞周期，同时以FCM检测Caspase-3、P-GP蛋白表达及细胞内药物积聚能力，RT-PCR法检测mdr-1基因表达。结果小檗胺能抑制人白血病K562／Adr细胞生长且呈剂量依赖关系，并能诱导细胞凋亡，使Caspase-3蛋白表达及细胞药物外排能力增加，同时降低mdr-1基因mRNA和蛋白表达水平。结论小檗胺能激活Caspase-3以诱导人白血病K562／Adr细胞凋亡，同时能通过降低mdr-1表达逆转多药耐药。

Study on Effect of Berbamine on Multidrug Resistance Leukemia K562/Adr Cells

Objective To study the effect and mechanism of berbamine on the apoptosis of multidrug resistant leukemia K562/Adr cells and in reversing the drug resistance. Methods IC 50 value of K562/Adr cell was determined with MTT method, cell apoptosis rate was analyzed by flow cytometry with Annexin V FITC PI assay, with the peak and cell cycle detected by PI staining. At the same time, flow cytometry was also used in determining Caspase 3, P GP protein expression and drug accumulating capacity in cells, and RT PCR method was used to analyze the gene expression of mdr 1. Results Berbamine could inhibit human leukemia K562/Adr cell growth in dose dependent manner, it could also induce cell apoptosis, increase the protein expression of Caspase 3 and the drug excretion capacity of cells, reduce the mRNA and protein expression levels of mdr 1 gene. Conclusion Berbamine could activate Caspase 3 to induce human leukemia K562/Adr cell apoptosis, and by reducing mdr 1 gene expression to reverse its multidrug resistance.