首页 | 本学科首页   官方微博 | 高级检索  
检索        

当归补血汤对大鼠肝纤维化与肝脏脂质过氧化的影响
引用本文:陈园,陶艳艳,李风华,陈高峰,周滔,刘成海.当归补血汤对大鼠肝纤维化与肝脏脂质过氧化的影响[J].中国中西医结合杂志,2008,28(1):39-42.
作者姓名:陈园  陶艳艳  李风华  陈高峰  周滔  刘成海
作者单位:1. 上海中医药大学附属曙光医院上海市中医药研究院肝病研究所,上海,201203
2. 上海中医药大学科技实验中心
基金项目:上海市教委资助项目(No.006CZ005);上海高校中医内科学E研究院建设计划(No.E03008);上海市重点学科建设项目(No.Y0302)部分资助
摘    要:目的观察当归补血汤对大鼠肝纤维化的作用及其影响肝脏脂质过氧化的抗肝纤维化作用机制。方法Wistar雄性大鼠采用四氯化碳(CCl4)皮下注射及高脂低蛋白饮食复合因素诱导复制大鼠肝纤维化模型。模型大鼠随机分为模型组(14只),当归补血汤组(14只);另设正常组(10只)。自造模之日起,当归补血汤组以6 g/kg的大鼠体重剂量灌胃,每日1次,共6周。正常组与模型组灌胃以等量生理盐水。HE染色观察肝组织炎症与脂肪变性,天狼猩红染色观察肝组织胶原沉积。试剂盒法测定血清肝功能指标,包括丙氨酸转氨酶(ALT)与天冬氨酸转氨酶(AST)水平、总胆红素(TBil)与白蛋白(Alb)含量。生化法测定肝组织甘油三脂(TG)与丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,Jamall氏法测定肝组织羟脯氨酸(hydroxyproline,Hyp)含量,Western印迹法分析肝组织Ⅰ型胶原蛋白表达。结果与正常大鼠比较,模型大鼠血清ALT、AST水平及TBil含量明显升高,Alb含量明显降低;肝组织脂肪变性与胶原沉积明显,TG与MDA含量增加,SOD活性降低(均P<005)。当归补血汤组大鼠肝组织脂肪变性与胶原病理沉积显著改善;血清ALT、AST水平及TBil含量降低,血清Alb含量升高;肝组织Hyp、TG与MDA含量降低,SOD活性提高(均P<005)。结论当归补血汤具有良好的抗实验性大鼠肝纤维化作用,其主要作用机制与抗肝脏脂质过氧化损伤有关。

关 键 词:当归补血汤  肝纤维化  肝脏脂质过氧化
修稿时间:2007年8月15日

Effects of Danggui Buxue Decoction on Liver Fibrosis and Hepatic Lipid Peroxidation in Rats
Authors:CHEN Yuan  TAO Yan-yan  LI Feng-hua
Institution:Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai.
Abstract:OBJECTIVE: To observe the effects of Danggui Buxue Decoction (DBD) on liver fibrosis and to explore its mechanism related to hepatic lipid peroxidation in rats. METHODS: Liver fibrosis model was established in 28 rats by the combination of injecting carbon tetrachloride (CCl4) subcutaneously and feeding high lipid and low protein diet. The model rats were randomly divided into 2 groups, the model group (n = 14) and the treated group (n = 14). Besides, a normal group was set up with 10 normal rats. For the treated group, rats were administered with DBD at a dosage of 6 g/kg body weight once a day by gastrogavage starting from the day of modeling for 6 successive weeks and to the control group, equal volume of normal saline was administered instead. The inflammation and fatty degeneration in rat liver tissues were examined with HE staining; the collagen deposition observed with sirius red; the liver function including serum level of alanine transaminase (ALT), aspartate aminotransferase (AST), albumin (Alb) and total bilirubin (TBil) were determined using corresponding test kits; the hepatic lipid peroxidation indexes, including triglyceride (TG), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by biochemical methods; the hepatic hydroxyproline (Hyp) content was detected with Jamall's method and the expression of collagen type I was analyzed by Western blotting. RESULTS: Compared with those in the normal rats, serum ALT, AST, TBil level, TG and MDA content remarkablely increased, level of Alb and SOD activity decreased, and hepatic fatty degeneration and collagen pathological deposition in liver was more obvious in the model rats (all P < 0.05). While in the DBD group, the hepatic fatty degeneration and collagen deposition were significantly improved, changes of all the above-mentioned indexes were significantly reversed (P <0.05). CONCLUSION: DBD has a good antagonist effect against experimental liver fibrosis, and its mechanism may be related to the anti-lipid peroxidation injury effect.
Keywords:Danggui Buxue Decoction  liver fibrosis  hepatic lipid peroxidation
本文献已被 维普 万方数据 等数据库收录!
点击此处可从《中国中西医结合杂志》浏览原始摘要信息
点击此处可从《中国中西医结合杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号