黄芪提取物免疫调节活性的体外实验研究 |
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引用本文: | 王润田,单保恩,李巧霞,唐建发,乔芳,杜肖娜,李宏,叶静.黄芪提取物免疫调节活性的体外实验研究[J].中国中西医结合杂志,2002,22(6):453-456. |
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作者姓名: | 王润田 单保恩 李巧霞 唐建发 乔芳 杜肖娜 李宏 叶静 |
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作者单位: | 1. 河北医科大学基础研究所免疫室,石家庄,050017 2. 河北医科大学第四医院科研中心 |
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基金项目: | 河北省教育厅攻关课题基金 (No .2 0 0 0 1 1 2 ) |
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摘 要: | 目的:研究黄芪提取物(Astragalus membranaceus extract,AME)对人的外周血免疫细胞(PBIC)免疫功能的调节作用。方法:采用^3H-TdR掺入法分析AME对外周血单个核细胞(PBMC)的增殖活性及对外周血粘附单核细胞(PBAM)吞噬肿瘤细胞的影响;采用^51Cr释放法测定AME对杀伤性T细胞(CTL)杀伤肿瘤细胞的影响;用ELISA法和生物学法研究AME对外周血B细胞(PBBC)产生IgG及对PBAM产生细胞因子的影响;采用SDS-PAGE分析AME的蛋白组成。结果:AME能促进PBMC的增殖;提高CTL对肿瘤细胞的杀伤活性;增强PBAM对肿瘤细胞的吞噬和产生细胞因子的功能;促进了PBBC产生IgG;AME含有多种蛋白成分。结论:AME对人免疫功能有增强作用,提高了抗肿瘤免疫效应,可应用于临床调节免疫功能和治疗肿瘤等疾病。
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关 键 词: | 黄芪提取物 免疫调节 实验研究 |
修稿时间: | 2001年6月25日 |
Extracorporeal Experimental Study on Immuno-Modulatory Activity of Astragalus memhranaceus Extract |
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Authors: | WANG Run tian SHAN Bao en LI Qiao xia |
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Abstract: | OBJECTIVE: To study the effect of Astragalus membranaceus extract (AME) in regulating the immune function of human peripheral blood immune cells (PBIC) in vitro. METHODS: Effects of AME on the proliferation activity of peripheral blood mononuclear cells (PBMC) and the tumor cell phagocytosis of peripheral blood adherent monocytes (PBAM) were measured by using 3H-TdR incorporation. Effect of the tumor-killing activity of cytotoxic T-lymphocyte (CTL) was determined by using 51Cr-releasing assay. Effects on production of IgG by peripheral blood B cells (PBBC) and IL-6 by PBAM were tested by means of ELISA, and effect on production of TNF-alpha by PBAM was studied by means of biological method. Besides, the protein elements of AME were analysed by SDS-PAGE. RESULTS: AME could promote the proliferation of human PBMC, elevate the tumor cell-killing activity of CTL, strengthen the tumor cell phagocytosis and cytokines (TNF-alpha and IL-6) production of PBAM, and promote the IgG production of PBBC. AME contained multiple protein elements. CONCLUSION: AME has effect in enhancing human immuno-function and anti-tumor activity, it could be applied in clinical practice for immuno-modulation and tumor treatment. |
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Keywords: | Astragalus membranaceus extract immuno modulation human peripheral blood immune cells immuno function |
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