身痛逐瘀汤对骨癌痛小鼠痛行为及脊髓星形胶质细胞活化的影响

目的 探讨身痛逐瘀汤的镇痛作用及对脊髓水平胶质细胞纤维酸性蛋白（GFAP）表达的影响。

Effect of Shentong Zhuyu Decoction on Pain Behavior and Spinal Cord Astrocytes Activation in a Mouse Model of Osteocarcinoma Pain

Objective To study the analgesic effect of Shentong Zhuyu Decoction（SZD） and its effect on the expression of the spinal cord glial fibrillary acidic protein（GFAP）.Methods One hundred C3H/HeNCrlVr male mice were randomly divided into the normal group（n=8）,the sham operation group（n=30）,the model group（n=30）,the Chinese medicine（CM） group 1（n=8）,the CM group 2（n=8）,the CM group 3（n=8）,and the vehicle group（n=8）.0.1 g crude drug of SZD/0.4 mL,0.3 g crude drug of SZD/0.4 mL,0.9 g crude drug of SZD/0.4 mL,and 0.4 mL normal saline were respectively given by gastrogavage to mice in CM 1,2,3 groups and the vehicle group,once daily for seven days starting from Day 14.The paw withdrawal thermal latency（PWTL）,as the behavior indicator,was assessed in mice using radiant thermal stimulator.The lumbar enlargement of the spinal cord was taken after the behavioral test on Day 21.GFAP mRNA and protein expressions were detected using Real-time quantitative RT-PCR and Western blot.Results Compared with the normal group（Day 0）（PWTL：15.91 ± 1.65 s） and the sham operation group（PWTL：Day 4：13.33±1.44 s;Day 7：11.28±0.61 s;Day 10：15.47±2.46 s;Day 14：15.69±1.98 s;Day 21：15.69±1.68 s）,the PWTL value in the model group（Day 4：13.24±1.02 s;Day 7：11.30±1.09 s;Day 10：9.12±0.54 s;Day 14：7.79 ± 0.77 s;Day 21：6.36±0.59 s） progressively decreased（P0.05） as time went by,while the spinal cord GFAP mRNA and protein expressions gradually increased.Compared with the normal group（Day 0） and the sham operation group（Day 14）,the PWTL value in the CM groups and the vehicle group obviously decreased on Day 14（P0.05）.The PWTL value was not significantly different among the model group,CM groups,and the vehicle group on Day 14（P0.05）.On Day 21 the PWTL value of CM group 2 and 3 increased and the spinal cord GFAP mRNA and protein expression levels decreased when compared with the model group and the vehicle group（P0.05）.But no significant difference in the PWTL value or GFAP expression levels was shown among the CM 1 group,the vehicle group,and the model group（P0.05）.Conclusion SZD had analgesic effect.Inhibition of the proliferation and activation of the spinal cord astrocytes might be one of its mechanisms.