慢性乙型肝炎中医证型与HLA-DRB1位点表达探讨

目的探讨慢性乙型肝炎（慢乙肝）中医证型与人类白细胞抗原-DRBl（HLA-DRBl）表达是否存在内在联系。方法用常规方法提取血液中白细胞DNA并用PCR方法对其相关片断进行扩增，用HLA基因分型芯片对HLA-DRBl位点进行分型检测。结果健康对照组有HLA-DRBl＊13表达，但在慢乙肝患者组未见其表达，两组比较，差异有显著性（P〈0．05）；慢乙肝患者分型有湿热中阻、肝郁脾虚、瘀血阻络、肝肾阴虚、脾肾阳虚，前三者为实证，后两者为虚证，慢乙肝辨证以实证居多。结论慢乙肝患者与健康对照者在HLA—DR某些位点表达上存在差异，这一差异的作用尚有待进一步证实，同时慢乙肝虚、实不同证型间HLA-DR基因位点表达可能存在不同。

Exploration on Relationship between TCM Syndrome Type and Expression of Human Leucocyte Antigen Locus-DRB1 in Patients with Chronic Hepatitis B

Objective To study the relationship between TCM syndrome type and expression of human leucocyte antigen-DRB1 (HLA-DRB1) in patients with chronic hepatitis B. Methods Using PCR method to amplify the related segments of DNA extracted from peripheral leucocytes by the routine methods,and gene array analysis was performed to detect the expression of HLA-DRB1. Results HLA-DRB113 was expressed in healthy person in the control group, but was not expressed in chronic hepatitis B patients, showing significant difference between the two groups. In the patients with five different syndrome types, i.e. the dampness blocking middle-jiao type (A), the Gan-stagnancy with Pi-deficiency type (B), the blood stasis blocking collaterals type (C), the Gan-Shen yin-deficiency type (D) and the Pi-Shen yang-deficiency type (E), the former three belonged to the excessive syndrome and the latter two were deficient syndrome. Most of the CHB patients were differentiated as excessive syndrome. Conclusion Difference of HLA-DR expression exists between chronic hepatitis B patients and healthy persons, the action of the difference is remained for further confirmation. HLA-DR expression in patients with different syndrome types, excessive or deficient, might be different, too.