茯苓水提液对新生大鼠神经细胞内钙离子浓度的影响

探讨茯苓水提液对神经细胞内钙稳态的影响。应用双波长荧光分光光度计和激光共聚焦扫描显微镜测定新生大鼠大脑皮层神经细胞悬液和海马神经元原代培养在加入茯苓水提液后细胞内钙离离子浓度的变化。

Effect of Water Extract of Poria on Cytosolic Free Calcium Concentration in Brain Nerve Cells of Neonatal Rats

OBJECTIVE: To investigate the effect of Poria cocos, a Chinese medicinal herb, on cytosolic free calcium concentration (Ca2+]i) in brain nerve cells of neonatal rats. METHODS: Double-wave-length fluorospectrophotometer and laser confocal scanning microscope were used to measure the Ca2+]i in neurons. RESULTS: The water extract of poria (31-250 mg/L) induced a dose-dependent increase in Ca2+]i, but poria at the range of 500 mg/L did not cause Ca2+]i to increase. It was also shown that the water extract of poria significantly inhibited the increment of Ca2+]i induced by 500 mumol/L glutamate. The similar results were observed from the experiments in primarily cultured hippcampal neurons of neonatial rats by using laser confocal scanning microscope. CONCLUSIONS: The water extract of Poria cocos has the effect of bidirectional regulation on cytosolic free calcium in brain nerve cells.