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不同舌苔舌上皮细胞的凋亡及相关基因分子机理研究
引用本文:吴正治,李明,张盛薇,蔡英,张永锋,陈嫚茵.不同舌苔舌上皮细胞的凋亡及相关基因分子机理研究[J].中国中西医结合杂志,2005,25(11):986-988.
作者姓名:吴正治  李明  张盛薇  蔡英  张永锋  陈嫚茵
作者单位:1. 广东省深圳市中西医结合临床研究所,广东518031
2. 广东省深圳市中医院
3. 深圳市第二人民医院口腔科
摘    要:目的 探讨不同舌苔舌上皮细胞凋亡及其相关基因表达的关系.方法 运用末端脱氧核苷酸转移酶介导的脱氧尿嘧啶核苷三磷酸缺口末端标记技术、原位杂交、免疫组化和图像分析技术,检测常见舌苔舌上皮细胞凋亡及凋亡相关基因bax、fas、TGF-β3 mRNA和蛋白产物.结果 4种常见舌苔其舌上皮细胞均可见细胞凋亡发生,不同舌苔变化趋势与凋亡指数变化趋势相反.与正常及薄苔比较,剥苔bax、fas基因过度表达伴随细胞凋亡增多,而厚苔bax、TGF-β3 mRNA低表达伴随细胞凋亡减少.舌苔上皮细胞中促凋亡基因bax、fas、TGF-β3表达水平变化趋势与细胞凋亡水平变化趋势一致.结论 凋亡相关基因bax、fas、TGF-β3表达水平的变化可能是影响舌苔上皮细胞凋亡,并导致不同舌苔变化的重要原因.

关 键 词:舌苔  原位缺口末端标记技术  原位杂交  免疫组织化学  图像分析
收稿时间:2005-04-02
修稿时间:2005-06-20

Study on the Molecular Mechanism of Lingual Epithelial Cell Apotosis and its Related Genes in Different Tongue Furs
Authors:WU Zheng-zhi  LI Ming  Sheng Wei
Institution:Clinical Institute of Integrative Traditional Chinese and Western Medicine of Shenzhen City, Guangdong 518031
Abstract:OBJECTIVE: To investigate the molecular mechanism ot lingual epithelial cell (LEC) apoptosis and its related genes expression in different tongue furs. METHODS: The LEC apoptosis and its related genes expression including bax, fas, TGF-beta 3 mRNA and protein product in tongue fur was determined using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling technique (TUNEL), in situ hybridization, immunohistochemical technique and image analysis. RESULTS: LEC apoptosis could always be seen in 4 types commonly encountered tongue fur. The tendency of changing in thickness of tongue fur was opposite to that of apoptotic index. Compared with normal thin fur, bax and fas genes were over-expressed in exfoliative fur with increased apoptosis, while in thick fur, bax and TGF-beta 3 genes were low-expressed and accompanied with decreased apoptosis. The level of apoptosis promoting genes, bax, fas, TGF-beta 3 gene expression in LEC showed a tendency parallel to that of LEC apoptosis. CONCLUSION: Change of apoptosis related genes expression, bax, fas and TGF-beta 3 may effect the LEC apoptosis and be the important factor for changing of the thickness of tongue fur.
Keywords:tongue fur  TUNEL  in situ hybridization  immunohistochemical  image analysis
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