实验性大鼠肺血栓栓塞症中ICAM-1、P-选择素的变化及红花注射液对其的影响

目的动态观察红花注射液对大鼠肺血栓栓塞症（PTE）中血管内皮细胞间黏附分子-1（ICAM-1）和P-选择素的影响.方法将Wistar大鼠随机分为正常组、模型组和治疗组,用颈静脉注入自体血凝块制备动物模型.治疗组在模型建立后立即自鼠尾静脉注射红花注射液2 ml/kg,每天1次,共5天.各组大鼠分别于栓塞后1、3、24、72、120 h处死,行病理观察;采用免疫组化和原位杂交的方法检测肺血管内皮ICAM-1和P选择素蛋白及mRNA的表达水平.结果与正常组比较,模型组大鼠肺栓塞后病理可见肺动脉血栓形成,炎性反应明显;肺血管内皮ICAM-1蛋白及mRNA表达在栓塞后3 h开始增高（P＜0.01）,而P-选择素蛋白及mRNA表达在栓塞后1 h开始增高（P＜0.01）.治疗组大鼠病理损伤减轻,肺血管内皮ICAM-1和P-选择素水平均下降（P＜0.01）.结论红花注射液可减轻大鼠PTE时的肺损伤,这种作用可能是通过下调血管内皮上的黏附分子来实现的.

Changes of ICAM-1 and P-selectin in Rats with Pulmonary Thromboembolism and the Effect of Safflower Injection

OBJECTIVE: To observe dynamically the expressions of intercellular adhesion molecule-1 (ICAM-1) and P-selectin (Ps) in the vascular endothelium in rats with pulmonary thromboembolism (PTE) and the effect of Safflower Injection (SI). METHODS: Rats were randomly divided into the normal group, the model group and the treatment group. PTE model was induced by intravenous injection of auto-blood clots, and SI was injected intravenously immediately after modelling with 2 ml/(kg d) for 5 days to the rats in the treatment group. Animals were sacrificed in batches at the 1st, 3rd, 24th, 72nd and 120th h after embolization to observe the pathological changes and detect the protein and mRNA expressions of ICAM-1 and Ps in pulmonary vascular endothelium by immunohistochemistry and in situ hybridization respectively. RESULTS: Pathological observation showed obvious embolism in pulmonary arteries and inflammatory reaction after modelling, which was abated after SI treatment. ICAM-1 and Ps expressions were elevated at the 3rd and the 1st h after embolization respectively (both P < 0.01), which also were abated in the treatment group. CONCLUSION: SI may alleviate pulmonary injury in PTE rats by down-regulating the expressions of ICAM-1 and Ps.