腹水草抗大鼠乙醇型胃溃疡的作用及机制研究

目的 研究腹水草水提液抗大鼠乙醇型胃溃疡的作用及机制。方法 48只健康SD大鼠随机分成正常组、模型组、雷尼替丁组，以及腹水草高、中、低剂量组，每组8只。正常组和模型组给予生理盐水灌胃；雷尼替丁组给予0.18%雷尼替丁混悬液［0.027 g/（kg·d)］灌胃；腹水草各剂量组分别给予高、中、低剂量腹水草水提液［2.8、1.4、0.7 g/（kg·d)］灌胃，每天1次，连续14天。于最后1次灌胃后，采用无水乙醇灌胃造模。比较各组大鼠胃溃疡指数及溃疡抑制率，检测血清及胃黏膜组织丙二醛(MDA)、NO含量和超氧化物歧化酶(SOD)活性，以及组织表皮生长因子(epidermal growth factor, EGF)含量。结果 腹水草各剂量组及雷尼替丁组溃疡指数较模型组明显减小(P<0.01)，且腹水草各剂量组呈剂量依赖性(P<0.01)；腹水草高剂量组的溃疡抑制率最高(P<0.01)。与正常组比较，模型组大鼠血清和组织MDA、NO含量显著升高，SOD活性以及组织EGF含量显著降低(P<0.01)。与模型组比较，腹水草各剂量组及雷尼替丁组血清和组织MDA含量降低，血清NO含量升高，组织NO下降，血清SOD活性升高，组织EGF含量提高，差异均有统计学意义(P<0.05，P<0.01)。结论 腹水草水提液具有抗大鼠乙醇型胃溃疡的作用。减少自由基生成、促进氧自由基清除及抗脂质过氧化反应，调控机体中NO和EGF含量，可能是其作用机制。

Antiulcer Effects and Mechanism Study of Veronicastrum axillare on Ethanol Induced Gastric Ulcer Rats

Objective To study the antiulcer effects and the mechanism of Veronicastrum axillare (Sieb. et Zucc) Yamazaki (VAY) on ethanol induced gastric ulcer rats. Methods Totally 48 healthy SD rats were randomly divided into 6 groups, i.e., the normal group, the model group, the ranitidine group, the high dose VAY group, the medium dose VAY group, and the low dose VAY group, 8 in each group. Rats in the normal group and the model group were administered with normal saline respectively. Rats in the ranitidine group were administered with 0.18% ranitidine suspension (at the daily dose of 0.027 g/kg) by gastrogavage. Those in the high dose VAY group, the medium dose VAY group, and the low dose VAY group were administered with VAY at the daily dose of 2.8 g/kg, 1.4 g/kg, and 0.7 g/kg by gastrogavage, once daily for 14 consecutive days. The gastric ulcer model was established using absolute ethanol after the last gastrogavage. The ulcer index and the ulcer inhibitory rate were compared. The concentrations of malonyldialdehyde (MDA), nitric oxide (NO), epidermal growth factor (EGF), and the activity of superoxide dismutase (SOD) in the serum and the homogenate of the gastric mucosa tissue were detected. Results Compared with the model group, the gastric ulcer index in the rest groups obviously decreased (P<0.01). The ulcer index was dose-dependent with VAY (P<0.01), with the highest gastric ulcer index shown in the high dose VAY group (P<0.01). Compared with the normal group, the concentrations of MDA and NO significantly increased in the serum and the gastric mucosa tissue, the activity of SOD and the EGF content in the gastric mucosa tissue of rats in the model group significantly decreased (P<0.01). Compared with the model group, the MDA concentrations in the serum and the gastric mucosa tissue decreased, the serum NO content increased, the NO content in the gastric mucosa tissue decreased, the serum SOD activity increased, the EGF content in the gastric mucosa tissue increased in the rest groups, all showing statistical difference (P<0.05, P<0.01). Conclusions The water extract of VAY had significant effects on ethanol induced gastric ulcer. Its mechanisms might lie in reducing the generation of free radicals, promoting the oxygen free radical clearance, restraining lipid peroxidation, regulating and controlling the in vivo contents of NO and EGF.