中医通里攻下法对多器官功能不全综合征时肠道屏障功能保护作用的实验研究

目的:探讨中医通里攻下法对多器官功能不全综合征(MODS)时肠道屏障功能的保护作用。方法:采用酵母多糖腹腔注射制备大鼠MODS模型。应用通里攻下中药大承气汤对模型动物进行治疗,并观察内毒素、二胺氧化酶(DAO)、黄嘌呤氧化酶(XOD)、过氧化脂质(MDA)、还原型谷胱甘肽(GSH)、肿瘤坏死因子(TNF)含量的变化,肠粘膜、肠细胞膜通透性及小肠粘膜病理形态学改变。结果:MODS时肠道屏障功能明显受到损伤和破坏。大承气汤能明显降低MODS大鼠血中内毒素水平,减少了血液和肠组织中TNF的含量,降低了血和肠组织中XOD和MDA水平,提高了GSH的含量,显著提高了肠组织中DAO的含量,并能降低模型动物肠粘膜和肠细胞膜通透性,减轻肠粘膜组织的病理损害。结论:通里攻下中药对MODS时肠道屏障功能具有明显的保护作用。

Protective Effects of Tongli Gongxia Herbs on Gut Barrier in Rat with Multiple Organ Dysfunction Syndrome

To explore the protective effects of Tongli Gongxia (TLGX) herbs on gut barrier with multiple organ dysfunction syndrome (MODS). Methods: The MODS models were induced by intraperitoneal injection of Zymosan A, and Dachengqi decoction (DCQD) was used in treating MODS models. The levels of endotoxin in the peripheral and portal blood, and the contents of diamine oxidase (DAO), xanthine oxidase (XOD), malondialdehyde (MDA), reduced glutathione (GSH) and tumor necrosis factor (TNF), the permeability of intestinal mucosa and enterocytes, and the histopathologic changes were observed. Results: The gut barrier function in MODS rats were severely damaged. DCQD could significantly reduce the levels of endotoxin in the blood (P<0 01), the contents of XOD, MDA and TNF in the blood and intestinal tissue were lowered (P<0 01), but the levels of GSH in blood and intestinal tissue were raised (P<0 05). Levels of DAO in the intestinal tissue was markedly increased (P<0 01). DCQD could reduce the permeability of intestinal mucosa and enterocytes, and attenuate the histopathologic changes of intestinal mucosa in MODS rats. Conclusion: TLGX herbs have apparently protective effects on the gut barrier in MODS rats.