首页 | 本学科首页   官方微博 | 高级检索  
检索        

三氧化二砷对哮喘豚鼠肺内嗜酸性粒细胞凋亡的影响
引用本文:周林福,殷凯生.三氧化二砷对哮喘豚鼠肺内嗜酸性粒细胞凋亡的影响[J].中国中西医结合杂志,2002,22(4):292-294.
作者姓名:周林福  殷凯生
作者单位:南京医科大学第一附属医院呼吸内科,南京,210029
基金项目:江苏省中医管理局基金资助项目(No.9974)
摘    要:目的:研究三氧化二砷(As2O3)对哮喘豚鼠肺内嗜酸性粒细胞(EOS)凋亡的影响及其作用机制。方法:豚鼠30只随机分为3组,即对照组、哮喘组和As2O3(2mg/kg)治疗组。采用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿嘧啶缺口末端标记(TdT-mediated dUTP nick end labelling,TUNEL)技术原位标记细胞凋亡;计算机图像分析技术对气道壁EOS浸润和EOS凋亡进行定量测定。结果:对照组豚鼠气道壁EOS计数(4.4±2.5)个/HP,EOS凋亡指数为(0.42±0.08)%;与对照组比较,哮喘组豚鼠气道壁EOS浸润显著增加(P<0.01),EOS凋亡指数显著下降(P<0.01);As2O3治疗组,气道壁EOS浸润显著下降(P<0.01),EOS凋亡指数显著增加(P<0.01),EOS凋亡指数显著下降(P<0.01);As2O3治疗组,气道壁EOS浸润显著下降(P<0.01),EOS凋亡指数明显增加(P<0.01),并且气道壁EOS浸润数量与EOS凋亡指数之间呈显著性负相关(r=-0.949,P<0.01)。结论:EOS凋亡异常是支气管哮喘的一个重要的发病机制;As2O3通过促进肺内EOS凋亡,下调EOS浸润数量,从而减轻了哮喘气道炎症;小剂量As2O3治疗哮喘既有效又相对安全,具有潜在的应用价值。

关 键 词:细胞凋亡  三氧化二砷  哮喘  豚鼠  肺内嗜酸性粒细胞
修稿时间:2001年9月12日

Effect of Arsenic Trioxide on Apoptosis of Pulmonary Eosinophile in Asthmatic Guinea-Pigs
Authors:ZHOU Lin  fu and YIN Kai  sheng
Institution:First Hospital Affiliated to Nanjing Medical University, Nanjing 210029.
Abstract:OBJECTIVE: To study the effect and mechanism of arsenic trioxide (As2O3) on apoptosis of pulmonary eosinophiles (PE) in asthmatic guinea-pigs. METHODS: Thirty guinea-pigs were divided into 3 groups at random, the control group, the asthmatic group and the As2O3 group. The dosage of As2O3 used was 2 mg/kg. The apoptotic PE were labelled by TdT-mediated dUTP nick end labelling technique, and the PE infiltration and apoptosis were detected quantitatively using computerized image analysis technique. RESULTS: In the control group, the amount of infiltrating PE was 4.4 +/- 2.5 cells/HP and the PE apoptotic index (AI) was 0.42 +/- 0.08%. In the asthmatic group, the amount increased (P < 0.01) and AI decreased significantly (P < 0.01). After the asthmatic animals had been treated with As2O3, the two parameters changed reversedly significantly (P < 0.01), and there was a significantly negative correlation between them (r = -0.949, P < 0.01). CONCLUSION: The PE apoptosis abnormality is one of the important mechanisms that cause bronchial asthma, As2O3 could alleviate the airway inflammation through promoting PE apoptosis and lower PE infiltration. Low dose of As2O3 is proved to be effective with relative safety, it also has potential value in treating asthma.
Keywords:arsenic trioxide  bronchial asthma  eosinophile  cell apoptosis
本文献已被 维普 万方数据 等数据库收录!
点击此处可从《中国中西医结合杂志》浏览原始摘要信息
点击此处可从《中国中西医结合杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号