调肝、补肾、健脾方药对慢性心理应激大鼠单胺类神经递质影响的比较研究

目的：观察慢性心理应激反应大鼠血浆如下丘脑多巴胺（DA）、5－羟色胺（5－HT）、肾上腺素（E）、去甲肾上腺素（NE）的变化及调肝、补肾、健脾方药对其影响。方法：Wistar大鼠按体重随机分为6组，即对照组（C），模型组（M），调肝中药（加味四逆散）组（C1），人参总皂甙组9C2），补肾中药（肾气丸）组（C3），健脾中药（四君子汤）组（C4）。采用束缚限制活动空间的方法造成慢性心理应激大鼠模型，所有药物灌胃给药。采用电化学高效液相色谱分析方法检测血浆和下丘脑DA、5－HT、E、NE含量。结果：应激大鼠血浆和下丘脑DA、5－HT含量明显降低（均P＜0.01)，血浆NE、E含量明显升高（均P＜0.01)，而下丘脑NE含量无明显变化。血浆5－HT／NE、5－HT／E及下丘脑5－HT／NE均明显降低（均P＜0.01)。加味四逆散可以升高应激大鼠下丘脑和血浆中DA、5－HT含量（均P＜0.01)，并能降低血浆中NE、E含量（均P＜0.01)，升高下丘脑和血浆5－HT/NE以及血浆5－HT／E（P＜0.05或P＜0.01)。肾气丸、四君子汤及人参部 甙虽然对有些指标有影响，但对心理应激的整体调节作用不如加味四逆散。结论：加味四逆散对慢性心理应激大鼠血浆和下丘脑单胺类神经递质的代谢紊乱均有良好的调节作用，调肝、补肾、健脾治法方药中以调肝治法方药的综合调治作用最为显著。

Comparative Study on Effects of Liver-regulating, Kidney-tonifying and Spleen-invigorating Recipes on Monoamine Neurotransmitter in Plasma and Hypothalamus of Rats with Chronic Psychological Stress

To observe the changes of monoamine neurotransmitters including 5-hydroxytryptamine (5-HT), dopamine (DA), epinephrine (E) and nor-adrenalin (NE) in plasma and hypothalamus of rats with chronic psychological stress and the effects of Liver-regulating, Kidney-toriifying and Spleen-invigorating recipes on them. Methods: Wistar rats were randomly divided into 6 groups according to their body weight, the control group (C), the model group (M), the Liver-regulating group (treated with Jiawei Sinisan decoction), the total ginsenoside group, the Kidney-tonifying group (treated with Shenqiwan decoction) and the Spleen-invigorating group (treated with Sijunzi decoction) . The rat model of chronic psychological stress was established by bandaging and confining activity space. All the drugs were given through gastrogavage. The contents of monoamine neurotransmitters were detected by HPLC. Results: Levels of DA, 5-HT in hypothalamus and plasma lowered in the model rats significantly (P<0.01), levels of NE and E in plasma increased significantly (P<0.01) but that of NE in hypothalamus changed insignificantly, the 5-HT/NE and 5-HT/E in plasma and 5-HT/NE in hypothalamus lowered significantly (P<0.01). Jiawei Sinisan could improve these changes significantly (F< 0.01). Although the other 3 treatments showed somewhat influence on these criteria, the comprehensive effects of them was inferior to that of Jiawei Sinisan. Conclusion: Liver-regulating therapy with Jiawei Sinisan showed good regulatory effects on monoamine neurotransmitter metabolism in plasma and hypothalamus, which shows better effect than that of the other two (Kidney-tonifying and Spleen-invigorating).