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阿魏酸钠对糖尿病大鼠肾脏的保护作用及机制研究
引用本文:赵同峰,张梁,邓华聪.阿魏酸钠对糖尿病大鼠肾脏的保护作用及机制研究[J].中国中西医结合杂志,2004,24(5):445-449.
作者姓名:赵同峰  张梁  邓华聪
作者单位:1. 浙江大学附属第二医院内分泌科,杭州,310009
2. 浙江大学附属第二医院神经内科
3. 重庆医科大学附属第一医院内分泌科
摘    要:目的 探讨阿魏酸钠(SF)对糖尿病(DM)大鼠肾脏的保护作用及机制。方法 对链脲佐菌素(STZ)诱导的DM大鼠灌胃给予SF110mg/(kg·d),治疗8周,测定各组大鼠肾重/体重、血甘油三酯和胆固醇、肌酐清除率(Ccr)、24h尿蛋白、肾皮质内皮素-1(ET-1)和一氧化氮(NO)等,观察肾脏病理改变并用免疫组织化学方法检测肾组织转化生长因子-β1(TGF—β1)和Ⅳ型胶原的表达。并与正常组和DM模型组作比较。结果 与正常组比较,DM组大鼠肾重/体重、Ccr、24h尿蛋白、肾皮质ET-1显著升高(均P<0.01),肾脏病理学检查显著异常,TGF—β1和Ⅳ型胶原表达明显增高,SF组上述指标显著改善。结论 SF对DM大鼠肾脏具有保护作用,其机制可能与其减少肾脏ET-1的生成,抑制TGF—β1和Ⅳ型胶原的表达有关。

关 键 词:阿魏酸钠  糖尿病  大鼠  肾脏  SF  DM  作用机制  血糖
修稿时间:2003年12月3日

Renal Protective Effect and Its Mechanism of Sodium Ferulate in Diabetic Rats
Authors:ZHAO Tong-feng  ZHANGLiang and DENG Hua-cong
Abstract:OBJECTIVE: To study the renal protective effect of sodium ferulate (SF) and its mechanism in rats with diabetic mellitus (DM). METHODS: DM rats induced by streptozotocin were treated with SF 110 mg/kg per day for 8 weeks. The ratio of kidney weight/body weight (KW/BW), serum triglyceride (TG) and total cholesterol (TC), creatinine clearance rate (Ccr), urinary protein/24 hrs, levels of endothelin-1 (ET-1) and nitric oxide (NO) in renal cortex in rats were measured, the pathological change of kidney were observed and the expression of transforming growth factor-beta 1 (TGF-beta 1) and collagen IV (C-IV) in kidney were examined using immunohistochemical assay. The data obtained were compared with those obtained from untreated DM rats and normal rats respectively. RESULTS: Compared with the normal rats, in DM rats, Ccr, urinary protein/24 hrs, ET-1, expressions of TGF-beta 1 and C-IV were significantly increased in DM model rats (all P < 0.01), and significantly abnormal pathological change in kidney was found. While in the SF treated DM rats, the above-mentioned abnormal changes were all significantly improved. CONCLUSION: SF has effect in protecting kidney of DM rats, the mechanism might be related with its actions of reducing ET-1 production in kidney and inhibiting the expressions of TGF-beta 1 and C-IV.
Keywords:sodium ferulate  diabetes mellitus  diabetic nephropathy
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