动态温热疏密波对软骨细胞凋亡调控基因表达的影响

目的：探讨动态温热疏密波对骨性关节炎软骨细胞凋亡调控基因表达的影响。方法：大白兔60只，随机分为2组．即：正常组（10只）和手术组（50只）。手术组采用改良Hulth法复制膝骨性关节炎模型，术后随机分为5组，模型组造模后常规饲养，对照组微波治疗仪，实验1组电（疏密波）治疗，实验2组热（热软膜）治疗，实验3组电热（治疗型护膝动态温热疏密波）治疗，分别对应治疗16周后，观察关节软骨的组织形态学，检测软骨细胞的凋亡率及p21、p53、Bcl-2 mRNA的表达。结果：16周时，骨性关节炎模型成功建立，经治疗后各组关节软骨组织形态学病理变化程度明显轻于模型组（P〈0．01，P〈0．05）；各组软骨细胞的凋亡率、p53 mRNA表达，模型组、对照组、实验1组、实验2组、实验3组明显高于正常组（P〈0．01），对照组、实验2组、实验3组明显低于模型组、实验1组（P〈0．01，P〈0．05），实验3组明显低于对照组、实验2组（P〈0．01，P〈0．05）；软骨细胞p21、Bcl-2 mRNA表达，模型组、对照组、实验1组、实验2组、实验3组明显低于正常组（P〈0．01），对照组、实验2组、实验3组明显高于模型组、实验1组（P〈0．01，P〈0．05），实验3组明显高于对照组、实验2组（P〈0．01，P〈0．05）。结论：动态温热疏密波能有效下调软骨细胞p53 mRNA表达，上调p21、Bcl-2 mRNA表达，从而抑制软骨细胞的凋亡，延缓关节软骨退变。

The effect of dynamic warm sparse-dense wave on apoptosis regulatory genes expression of chondrocyte

Objective:To investigate the effect of dynamic warm sparse-dense wave on apoptosis regulatory genes expression of chondrocyte.Methods:60 white rabbits were randomly divided into normal group(n=10)and operation group(n=50).The operation group copied knee osteoarthritis by modified Hulth Law were randomly divided into 5 groups.Model group received conventional breeding after modeled,control group received the microwave therapy,experimental group 1 received electricity(sparse-dense wave) therapy,experimental group 2 received thermal(thermal soft film) therapy,and experimental group 3 received electricity and thermal(dynamic warm sparse-dense wave) therapy.After treatment 16 weeks,the morphology of articular cartilage was observed,apoptosis rate and p21,p53,Bcl-2 mRNA expression of chondrocyte was detected.Results:After 16 weeks,knee osteoarthritis model was successfully established,articular cartilage morphology in each treatment group level of pathological changes significantly reduced than its in model group(P<0.01,P<0.05).Apoptosis ate and p53 mRNA expression of chondrocyte,model group,control group,experimental group 1,experimental group 2 and experimental group 3 was significantly higher than the normal group(P<0.01),control group,experimental group 2 and experimental group 3 was significantly lower than the model group,experimental group 1(P<0.01,P<0.05),experimental group 3 was significantly lower than control group,experimental group 2(P<0.01,P<0.05);The expression of p21、Bcl-2 mRNA,Model group,control group,experimental group 1,experimental group 2,experimental group 3 was significantly lower than normal group(P<0.01),control group,experimental group 2 and experimental group 3 was significantly higher than model group,experimental group 1(P<0.01,P<0.05),experimental group 3 was significantly higher than control group,experimental group 2(P<0.01,P<0.05).Conclusion:Dynamic warm sparse-dense wave can be effect of down-regulating the expression of p53 mRNA,up-regulating the expression of p21、Bcl-2 mRNA,inhibit chondrocyte apoptosis and delay the degeneration of cartilage.