补肾法防治兔膝关节骨性关节炎的病理形态学观察

通过观察纯补肾药对兔膝骨性关节炎软骨及滑膜的影响，探讨骨性关节炎的发病机理及补肾法的可行性，以兔膝关节腔内注入木瓜蛋白酶造成骨性关节炎模型，用补肾中药灌骨治疗。造模６周后取材，大体观察后，切取膝前滑膜组织ＨＥ染色后光镜下观察，切取股骨内髁软一铅双重染色透射电镜观察及ＨＥ、Ｍａｓｓｏｎ三色，沙红－染色光镜下观察。结果示补肾组兔膝关节软骨细胞破坏芨滑膜组织损伤程度明显低于模型组，软骨胶原纤维紊乱及转型

THE PREVENTION AND TREATMENT OF RABBIT KNEE OSTEOARTHRITIS BY KIDNEY-TONIFY-ING METHOD:A Pathomorphological Observation

The authors explored the pathogenesis of osteoarthritis (OA) and the feasibility of the kidney--tonifying (KT) method through observing the influence of pure KT traditional Chinese drugs (TCD) on the cartilage and the synovium in rabbit knee OA. OA models were made by injecting papaininto rabbit knee articular cavity and treated by KT TCD given through the stomach. Six weeks later, the specimens were harvested for gross observation. Then. the femoral internal condyle was taken and observed under transmission electron microscope and under light microscope afterHE, Masson and Safranin-0 staining; the synovial tissue taken from the anterior knee was observed under light microscope after HE staining. Theresults showed that the degrees of the joint chondrocyte destruction and the synovial tissue injury of the rabbit knee in KT group were significantlylowered (P < 0. 10 ) and the disturbance and type-transformation of the chondral collagenous fibers were much alleviated in comparison with thoseof the model group. It was believed that KT TCD function to effectively prevent and treat experimental osteoarthritis, which verified the theory oftraditional Chinese medicine 'The kidneys govern the bone" again.