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| 火针对脊髓损伤模型大鼠IL-1β、Caspase-3蛋白表达的影响 | |
| 引用本文： | 周震,李岩,倪虹,孙立明,王宏业,李晓霞,张品,艾明媚,刘保红.火针对脊髓损伤模型大鼠IL-1β、Caspase-3蛋白表达的影响[J].上海针灸杂志,2010,29(5):318-321. |
| 作者姓名： | 周震 李岩 倪虹 孙立明 王宏业 李晓霞 张品 艾明媚 刘保红 |
| 作者单位： | 1. 天津中医药大学第二附属医院,天津,300150 2. 天津市公安医院,天津,300042 3. 南开大学,天津,300071 4. 天津中医药大学,天津,300193 |
| 基金项目： | 天津市应用基础及前沿技术研究计划重点项目 |
| 摘 要： | 目的探讨火针对脊髓损伤（SCI）模型大鼠Caspase-3、IL-1β蛋白表达的影响。方法选择SD大鼠80只,随机分为假手术组、模型组、火针组与毫针组,采用改良Allen＇s法建立急性SCI模型,经不同针刺方法干预后,在不同时段取材并通过免疫组化SABC法检测Caspase-3、IL-1β蛋白表达。结果模型组中,SCI大鼠脊髓内IL-1β与Caspase-3的蛋白表达明显增高;两个针刺干预组中,IL-1β与Caspase-3的蛋白表达明显降低,其中火针干预组更为明显。结论火针可降低SCI模型大鼠IL-1β、Caspase-3蛋白表达。 |
| 关 键 词： | 火针 脊髓损伤 IL-1β Caspase-3 蛋白表达 SABC法 大鼠 |
Effect of Fire Needling on Caspase-3 and IL-1β Protein Eexpressions in a Rat Model of Spinal Cord Injury |
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| ZHOU Zhen,LI Yan,NI Hong,SUN Li-ming,WANG Hong-ye,LI Xiao-xia,ZHANG Pin,AI Ming-mei,LIU Bao-hong.Effect of Fire Needling on Caspase-3 and IL-1β Protein Eexpressions in a Rat Model of Spinal Cord Injury[J].Shanghai Journal of Acupuncture and Moxibustion,2010,29(5):318-321. | |
| Authors: | ZHOU Zhen LI Yan NI Hong SUN Li-ming WANG Hong-ye LI Xiao-xia ZHANG Pin AI Ming-mei LIU Bao-hong |
| Institution: | ZHOU Zhen1,LI Yan2,NI Hong3,SUN Li-ming4,WANG Hong-ye4,LI Xiao-xia4,ZHANG Pin4,AI Ming-mei4,LIU Bao-hong2. 1.Second Hospital Affiliated to Tianjing University of Traditional Chinese Medicine,Tianjing 300150,China,2.Tianjing Public Security Hospital,Tianjing 300042,3.Nankai University,Tianjing 300071,4.Tianjing University of Traditional Chinese Medicine,Tianjing 300193 |
| Abstract: | Objective To explore the effect of fire needling on Caspase-3 and IL-1β protein expressions in a rat model of spinal cord injury （SCI）. Methods Eighty SD rats were randomly allocated to sham operation, model, fire needling intervention and filiform-needle acupuncture intervention groups. An acute SCI model was made by the modified Allen＇s method. The specimens were taken in different stages after the interventions of different acupuncture techniques. Caspase-3 and IL-1β protein expressions were examined by immunohistochemical SABC method. Results IL-1β and Caspase-3 protein expressions increased obviously in the model group of SCI rats. IL-1β and Caspase-3 protein expressions decreased obviously in the two acupuncture intervention groups. They decreased more obviously in the fire needling intervention group. Conclusion Fire needling can decrease IL-1β and Caspase-3 protein expressions in a rat model of SCI. |
| Keywords: | Caspase-3 |
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