糖脂平对糖耐量减低模型大鼠的治疗作用及其机理研究

目的探讨糖脂平对实验性糖耐量减低大鼠的治疗作用和对胰岛β细胞的影响。方法采用小剂量STZ（25mg/kg）腹腔注射联合高热量饲料喂养建立糖耐量减低大鼠模型,分为正常对照组、模型对照组、糖脂平组、联合（糖脂平＋阿卡波糖）组、阿卡波糖组;治疗4周后测2hPG,治疗8周后,检测血糖、血清胰岛素、游离脂肪酸,计算胰岛素敏感指数,进行胰岛细胞免疫组织化学分析。结果治疗4周后大鼠血糖开始下降,与模型对照组比较,联合组、阿卡波糖组大鼠血糖明显降低;治疗8周后,与模型对照组比较,联合组、阿卡波糖组、糖脂平组大鼠血糖、游离脂肪酸均明显降低,IAI明显升高;胰岛病理学观察结果显示联合组胰岛数量及胰岛内细胞数量较模型组明显增多,胰岛素阳性表达显著增强。结论中药复方糖脂平能够有效降低糖耐量减低模型大鼠的血糖、改善脂代谢紊乱,提高胰岛素敏感性,其机理可能为改善代谢,降低糖毒性、脂毒性,减少胰岛细胞凋亡,改善胰岛β细胞功能,增强组织对胰岛素的敏感性,从而有效发挥降低血糖的作用。

Effect and the Mechanism of Tangzhiping on Rats with Impaired Glucose Tolerance

Objective：To study the effect and the mechanism of Tangzhiping on impaired glucose tolerance rats and it on islet β cells.Methods：Rats models,induced by the peritoneal injection STZ and feeding high fatty and high sugar diet,were divided into the normal control group,the model control group,the Tangzhiping group,the combination group and the acarbose group.After 4 weeks,the 2hPG of rats were detected.After 8 weeks,the blood sugar,the serum insulin and the free fatty acid were detected.The insulin sensitivity index was calculated.Meanwhile,the islet cell immunohistochemistry was analysed.Results：After 4 weeks later,the blood sugar of rats were decreased.Compared with the model control group,this index of acarbose group was induced remarkably.After 8 weeks,the blood sugar and the free fatty acid of the combination group,the acarbose group and the Tangzhiping group were decreased significantly when they being compared with the model control group.Meanwhile,the IAI of above groups was increased obviously.In the combination group,the islet number and the islet inner cell were higher significantly than those in the model group.Also,the positive expression of the insulin in the combination group was more obviously than that in the model group.Conclusion：Tangzhiping can effectively reduce blood glucose,improve the blood grease and increase insulin sensitivity index of the impaired glucose tolerance rats.It mechanism is that Tangzhiping can improve metabolism,reduce sugar toxicity and lipid toxicity,reduce islet cell apoptosis,improve beta cell function and enhance organization to insulin sensitivity,and then bring into full play its pharmacodynamic action.