红景天对老年性痴呆大鼠行为学及海马区凋亡相关蛋白表达的影响

目的探讨红景天对老年性痴呆(AD)大鼠认知功能、海马CA1区Bcl-2、Bax蛋白表达的影响及其治疗阿尔茨海默病的可能作用机制。方法采用D-半乳糖 三氯化铝 氢溴酸东莨菪碱注射液法复制AD大鼠模型;采用Morris水迷宫法进行行为学检测,用免疫组化法检测海马CA1区Bcl-2、Bax蛋白的表达水平的变化。结果模型组与空白组相比,认知能力显著下降,Bcl-2/Bax比值下降;治疗组与模型组相比,认知能力显著改善,Bcl-2/Bax比值增加。结论红景天对AD大鼠脑神经细胞的保护作用可能是通过上调Bcl-2蛋白、下调Bax蛋白的表达,抑制细胞凋亡而实现,从而改善AD大鼠的学习记忆能力。

Effects of Rhodiola Herb Against Behavior and the Expression of Apotosis-related Proteins in the Hippocampus of Alzheimer's Disease in Rats

Objective:To study the effects of Rhodiola herb against the behavior and expression of Bcl-2 and Bax protein in hippocamps of Alzheimer's disease in rats.Methods:AD model was formed by adopting the D-gal ALCL3 SCOP in SD rats .Cognitive abilities of rats were studied with the Morris water maze.The expression of Bcl-2 and Bax protein in the hippocampus were studied with immunohistochemical staining.Results:AD model may decrease cognitive ability,the ratio of Bcl-2/Bax in the hippocampus comes down.Rhodiola herb significant alleviated cognitive ability,the ratio of Bcl-2/ Bax rises.Conclusion:Rhodiola herb can inhibit apoptosis by increasing the expression of Bcl-2 and reducing the expression of Bax protein in the hippocampus. Rhodiola herb may improve cognitive ability in AD rats.