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长春花叶片中吲哚生物碱增产的研究
引用本文:张秀省,张荣涛,聂莉莉,郭玉海,翟志席.长春花叶片中吲哚生物碱增产的研究[J].中草药,2005,36(2):265-267.
作者姓名:张秀省  张荣涛  聂莉莉  郭玉海  翟志席
作者单位:1. 中国农业大学农学与生物技术学院,北京,100094;聊城大学农学院,山东,聊城,252000
2. 南开大学生命科学学院,天津,300071
3. 中国农业大学农学与生物技术学院,北京,100094
摘    要:目的探讨乙烯利、乙酰水杨酸、色氨酸3种试剂对长春花植株中药用成分积累的影响。方法用不同质量浓度的3种试剂溶液分别处理温室盆栽长春花植株,2d后,测定叶片中的总吲哚生物碱含量,并采用RP-HPLC法测定长春碱和长春质碱的含量。结果3种试剂处理后吲哚总碱、长春碱、长春质碱的积累均有明显的提高,并确定了最适的处理质量浓度。结论3种试剂对长春花植物中吲哚生物碱的产生有明显的促进作用。

关 键 词:长春花  长春碱  乙烯利  乙酰水杨酸  色氨酸
文章编号:0253-2670(2005)02-0265-03
收稿时间:2004/4/12 0:00:00

Enhancing accumulation of indole alkaloids in Catharanthus roseus leaves
ZHANG Xiu-sheng,ZHANG Rong-tao,NIE Li-li,GUO Yu-hai and ZHAI Zhi-xi.Enhancing accumulation of indole alkaloids in Catharanthus roseus leaves[J].Chinese Traditional and Herbal Drugs,2005,36(2):265-267.
Authors:ZHANG Xiu-sheng  ZHANG Rong-tao  NIE Li-li  GUO Yu-hai and ZHAI Zhi-xi
Institution:ZHANG Xiu-sheng 1,3,ZHANG Rong-tao 2,NIE Li-li 2,GUO Yu-hai 1,ZHAI Zhi-xi
Abstract:Objective To study the effect of three kinds of reagent: ethrel, Aspirin, tryptophan on the accumulation of medicinal component in Catharanthus roseus leaves. Methods To treat these potplants of C. roseus in green house with the three reagents in different concentrations, respectively. Then, two days later, the contents of catharanthine and vinblastine in leaves were determined by RP-HPLC. Results The three reagents can obviously improve the accumulation of indole alkaloids, including catharanthine and vinblastine in C. roseus leaves. Their optimum treatment concentration was finally comfirmed. Conclusion These three kinds of reagent apparently promote the accumulation of total indole alkoloids in the leaves of C. roseus.
Keywords:Catharanthus roseus (L  ) G  Don  vinblastine  ethrel  Aspirin  tryptophan
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