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膜荚黄芪苯丙氨酸解氨酶基因的克隆与序列分析
引用本文:吴松权,祖元刚,管清杰,吴基日.膜荚黄芪苯丙氨酸解氨酶基因的克隆与序列分析[J].中草药,2010,41(3):456-460.
作者姓名:吴松权  祖元刚  管清杰  吴基日
作者单位:1. 延边大学农学院园艺系,吉林龙井,133400
2. 东北林业大学,黑龙江哈尔滨,150040
基金项目:国家自然科学基金资助项目(30860036)
摘    要:目的对膜荚黄芪苯丙氨酸解氨酶基因进行克隆及序列分析。方法应用RT-PCR和cDNA末端快速扩增法,以膜荚黄芪根总RNA为模板克隆PAL基因。结果所克隆的膜荚黄芪苯丙氨酸解氨酶基因命名为AmPAL,Genbank登录号为EF567076。序列分析表明,AmPAL全长为2650bp,含有一个完整的2154bp开放读码框。AmPAL是植物苯丙氨酸解氨酶家族的一个新成员,推测其编码718个氨基酸的多肽,相对分子质量为7.805×104,等电点为5.96,与豆科植物已知的PAL氨基酸序列的同源并且相似性都大于80%。结论首次成功地从膜荚黄芪中克隆出了PAL基因,为有效利用该基因调控药用植物苯丙烷代谢途径奠定了基础。

关 键 词:膜荚黄芪  苯丙氨酸解氨酶  基因克隆  
收稿时间:2009/5/12 0:00:00

Cloning and sequence analysis of a novel phenylalanine ammonia-lyase gene from Astragalus membranaceus
WU Song-quan,ZU Yuan-gang,GUAN Qing-jie and WU ji-ri.Cloning and sequence analysis of a novel phenylalanine ammonia-lyase gene from Astragalus membranaceus[J].Chinese Traditional and Herbal Drugs,2010,41(3):456-460.
Authors:WU Song-quan  ZU Yuan-gang  GUAN Qing-jie and WU ji-ri
Institution:WU Song-quan1,ZU Yuan-gang2,GUAN Qing-jie2,WU ji-ri1(1. Department of Horticulture,Agricultural College of Yanbian University,Longjing 133400,China,2. Northeast Forestry University,Harbin 150040,China)
Abstract:Objective To clone and sequence the cDNA encoding phenylalanine ammonia-lyase(PAL)gene from Astragalus membranaceus.Methods RT-PCR and RACE Techniques were used to clone a phenylalanine ammonia-lyase gene from A.membranaceus roots with the total RNA as the template.Results The cloned gene named as AmPAL and the Genbank registry number is EF567076.Squence analysis showed that the full-length of AmPAL cDNA was 2 650 bp,including a 2 154 bp open reading frame(ORF).AmPAL was a new number of PAL family that cons...
Keywords:Astragalus membranaceus(Fisch  )Bunge  phenylalanine ammonia-lyase  gene cloning  
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