基于ISSR的连翘遗传多样性研究

目的研究不同居群连翘的遗传多样性。方法采用ISSR分子标记技术对连翘25个自然居群的样本进行分析,利用POPGENE32软件分析多态位点百分率(P)、Nei’s基因多样性指数(H)及Shannon多样性指数(I);居群间遗传一致度采用NTSYSpc-2.10软件进行聚类分析。结果选出13条ISSR引物,扩增出353条清晰条带,物种水平P为100%,H为0.252 3,I为0.394 0,遗传分化系数(Gst)为0.331 8,居群间基因流(Nm)为1.007 0,遗传距离0.031 0～0.155 5;NTSYSpc软件进行系统聚类,将25个聚群划分为2大类,7分组;连翘个体间进行聚类分析,195份连翘样品划分为2大类,5分组。结论连翘种群遗传多样性水平较高,但连翘种群间遗传距离与地理距离没有相关性,与生态因子和生长环境有关。

Genetic diversity of Forsythia suspensa based on ISSR

Objective To investigate the genetic diversity of Forsythia suspensa from different populations. Methods ISSR markers were used to analyze the genetic diversity of 25 populations of F. suspensa. Percentage of species level polymorphic bands (P), Nei''s genetic diversity index (H), and Shannon''s information index (I) of genetic information were calculated by POPGEN 32. UPGMA relationship dendrogram was clustered by NTSYS. Results Thirteen primers produced 353 bands and P was 100%. H and I were 0.252 3 and 0.394 0, and genetic differentiation coefficient (G_st) and gene flow (N_m) were 0.331 8 and 1.007 0 within the population levels. The genetic distance varied from 0.031 0 to 0.155 5. NTSYSpc software was used to cluster the system. A total of 25 clusters were divided into two categories and seven groups. Cluster analysis was conducted among Forsythia individuals. A total of 195 samples were divided into two categories and five groups. Conclusion The genetic diversity among the populations of F. suspensa is at higher level. However, the genetic distance among the Forsythia population is not related to the geographical distance, which is mainly dependent on the ecological factors and the growth environment.