黄芩素干预快速老化小鼠SAMP8肝脏组织的代谢组学研究

目的基于~1H-NMR代谢组学技术,研究黄芩素对快速老化小鼠SAMP8肝脏的保护作用并探索其作用机制。方法采用SAMP8小鼠作为快速老化模型,考察黄芩素(ig)对SAMP8小鼠肝脏的保护作用。小鼠随机分3组,对照组为SAMR1小鼠、模型组为SAMP8小鼠、给药组为SAMP8小鼠+黄芩素。对照组和模型组给予等量生理盐水,给药组给予200 mg/kg黄芩素。分别取各组小鼠肝脏组织,采用苏木精-伊红(HE)染色观察其肝组织损伤程度,并对肝脏组织进行~1H-NMR检测,结合多元统计分析探讨黄芩素抗衰老小鼠肝损伤的作用。结果肝脏指数以及HE染色结果显示,黄芩素能明显改善SAMP8小鼠的肝损伤情况。代谢组学分析共找到8个潜在生物标志物,主要涉及3条代谢通路,分别是丙氨酸、天冬氨酸、谷氨酸代谢,甘氨酸、丝氨酸、苏氨酸代谢和肌醇磷酸代谢。结论黄芩素对SAMP8小鼠的肝损伤具有保护作用,其机制涉及多靶点、多途径。

Metabolomics-based study of metabolic changes in liver tissues of senescence accelerated mouse prone 8 (SAMP8) treated with baicalein

Objective To study the protective effect and explore the mechanism of baicalein on the liver of senescence-accelerated mouse prone 8 (SAMP8) based on ¹H-NMR metabolomics. Methods The protective effect of baicalein (ig) on the liver of SAMP8 mice was investigated in the present study. The mice in control group was SAMR1, the mice in model group was SAMP8, the drug treatment group was SAMP8 + baicalein. The mice in control and model group were administrated with equal amount of normal saline, and the mice in drug treatment group were administrated with 200 mg/kg baicalein. The liver tissues of mice in each group were isolated, and the damage degree of liver tissue was determined by hematoxylin-eosin (HE) staining. ¹H-NMR combined with multivariate statistical analysis was used to investigate the mechanism of baicalein on liver damage in aging mice. Results Organ index and HE staining results showed that baicalein can significantly improve liver damage in SAMP8 mice. Eight potential biomarkers were found in hepatic metabolomics analysis, mainly involving three metabolic pathways:Alanine, aspartic acid and glutamate metabolism; Glycine, serine, threonine metabolism, and inositol phosphate metabolism. Conclusion The study of metabolites alterations in the liver tissue of SAMP8 mice would provide experimental evidence for anti-aging drug research.