| 藏药雪莲原植物水母雪莲及其混淆种类的ITS序列比较和分子鉴定 |
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| 引用本文： | 刘建全,陈之端,路安民.藏药雪莲原植物水母雪莲及其混淆种类的ITS序列比较和分子鉴定[J].中草药,2001,32(5):443-445. |
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| 作者姓名： | 刘建全 陈之端 路安民 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所,
2. 中国科学院植物研究所 |
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| 基金项目： | 中国科学院生物科学与生物技术特别支持费课题资助。 |
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| 摘 要： | 目的：比较藏药“雪莲”原植物水母雪莲及其混淆种类的ITS序列，为分子序列鉴定提供参照系统。方法：利用PCR扩增和ABI377自动测序。结果：水母雪莲不同居群间的序列相同，与其混淆种类之间存在一定的分子序列差异。结论：ITS序列可正确鉴定水母雪莲与其混淆种类。
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| 关 键 词： | 藏药 雪莲 ITS序列 分子鉴定 水母雪莲 混淆种类 |
| 文章编号： | 0253-2670(2001)05-0443-03 |
| 收稿时间： | 2000/10/2 0:00:00 |
| 修稿时间： | 2000年10月2日 |
Comparison on internal transcribed spacers (ITS) sequences of Tibetan medicine Saussurea medusa and its easily confusable species |
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| LIU Jianquan,CHEN Zhiduan and LU Anmin.Comparison on internal transcribed spacers (ITS) sequences of Tibetan medicine Saussurea medusa and its easily confusable species[J].Chinese Traditional and Herbal Drugs,2001,32(5):443-445. |
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| Authors: | LIU Jianquan CHEN Zhiduan and LU Anmin |
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| Institution: | LIU Jian quan 1,CHEN Zhi duan 2,LU An min 2 |
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| Abstract: | Object In order to identify the medicine at the molecular level, the internal transcribed spacers (ITS) of Saussurea medusa Maxim and its easily confusable species were sequenced Methods The double stranded DNA was amplified using PCR systems 9 600 kits and sequenced on an ABI 377 automated sequencer from both directions Results The ITS sequences of S medusa of different populations showed no variation, but there existed distinct variation between S medusa and its confusable species Conclusion ITS sequences can be used for the molecular authentication between S medusa and its confusable species |
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| Keywords: | Tibetan medicine Saussurea medusa Maxim internal transcribed spacers (ITS) sequences molecular authentication |
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