| 线粒体nad 1内含子2序列在石斛属植物分子鉴定中的应用 |
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| 引用本文： | 张婷,王峥涛,徐珞珊,周开亚.线粒体nad 1内含子2序列在石斛属植物分子鉴定中的应用[J].中草药,2005,36(7):1059-1062. |
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| 作者姓名： | 张婷 王峥涛 徐珞珊 周开亚 |
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| 作者单位： | 1. 中国药科大学,生药学研究室,江苏,南京,210038
2. 南京师范大学生命科学学院,生物资源技术江苏省重点实验室,江苏 南京 210097 |
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| 基金项目： | 国家自然科学基金资助项目(30171144) |
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| 摘 要： | 目的在兰科石斛属药用植物鉴定中应用新的分子标记。方法扩增并测定9种石斛属植物线粒体中NADH脱氢酶亚基1编码基因(nad 1)内含子2(in tron 2)的全长序列。结果比对后的nad 1 in tron 2序列长872bp,其中有17个变异位点,可以鉴别除粉花石斛D end robium lodd ig esii以外的8种植物。结论线粒体nad 1 in tron2序列可以作为一种新的分子标记用于石斛属植物的鉴定。
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| 关 键 词： | 石斛属 线粒体nad1intron2 分子标记 |
| 文章编号： | 0253-2670(2005)07-1059-04 |
| 收稿时间： | 2004/10/24 0:00:00 |
Application of mitochondrial nad 1 intron 2 sequences to molecular identification of some species ofDendrobium Sw. |
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| ZHANG Ting,WANG Zheng-tao,XU Luo-shan and ZHOU Kai-ya.Application of mitochondrial nad 1 intron 2 sequences to molecular identification of some species ofDendrobium Sw.[J].Chinese Traditional and Herbal Drugs,2005,36(7):1059-1062. |
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| Authors: | ZHANG Ting WANG Zheng-tao XU Luo-shan and ZHOU Kai-ya |
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| Institution: | ZHANG Ting~1,WANG Zheng-tao~ |
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| Abstract: | Objective Application of a new molecular marker to the identification of Dendrobium((Orchidaceae)) species.Methods Complete sequences of the mitochondrial nad 1 intron 2 for nine species of Dendrobium Sw.were amplified and determined.Results Seventeen variable sites were found in the aligned 872 bp of nad 1 intron 2 sequences.Eight of the nine Dendrobium species except D.loddigesii could be identified by the nad 1 intron 2 sequences.Conclusion The mitochondrial nad 1 intron 2 sequences could be used as a new molecular marker for the identification of Dendrobium species. |
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| Keywords: | Dendrobium Sw mitochondrial nad 1 intron 2 molecular marker |
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