基于物质组分分配研究钼对甘草酸积累的影响

目的 基于物质分配研究钼对药材中甘草酸积累的影响并探讨其机制.方法 采用盆栽蛭石的试验方法,以一年生的甘草移栽苗为实验材料,共设置4个钼质量浓度水平,分别为0、0.52、5.2、10.4 mg/L,其中0.52 mg/L为正常Hoagland营养液中钼的质量浓度.每周向盆内浇灌营养液,以达到处理的目的.分别在处理35、70、105 d取样,测定甘草中甘草酸的相对量和绝对量以及总糖、粗蛋白、粗纤维、粗脂肪、灰分等物质组分量.结果 钼对甘草酸的相对量影响不显著,但是对其绝对量的影响显著,施钼可以提高甘草中总糖、粗蛋白和灰分的量以及5种主要物质组分量总和,降低粗脂肪的量,对粗纤维的影响不显著.甘草酸相对量与粗蛋白为显著正相关.甘草酸绝对量与粗纤维为极显著负相关,与灰分、粗脂肪和物质总和为极显著正相关,与总糖为显著正相关.结论 甘草酸的积累与粗纤维、粗脂肪、灰分、总糖的分配密切相关,适当质量浓度的钼可以刺激甘草内的总糖、粗脂肪等物质的合成,进而促进甘草的次生代谢,导致甘草酸的形成和积累.

Effect of molybdenum on accumulation of glycyrrhizic acid based on material ingredients distribution

Objective To investigate the effects of molybdenum (Mo) on the accumulation of glycyrrhizic acid (GA) in medicinal materials based on the material ingredients distribution and explore the mechanism. Methods The transplants of one-year-old G. uralensis were subjected to Mo at four concentration (0, 0.52, 5.2, and 10.4 mg/L) culturing in vermiculite, among which 0.52 mg/L was used as the normal concentration in the complete Hoagland nutrition solution. The solution was irrigated to the pots every week. The relative and absolute contents of GA, and the contents of total sugar, crude protein, crude fiber, crude fat, and ash were determined on the day 35, 70, and 105 after treatment, respectively. Results The influence of Mo on the relative contents of GA was not significant while significant to the absolute content of GA. The application of Mo could increase the contents of total sugar, crude protein, ash, and the total material ingredients, but decrease the contents of crude fat. There is no difference in the crude fiber. The relative content of GA had significant positive correlation with the crude protein. The absolute content of GA had extremely significant negative correlation with the crude fiber, and it had extremely significant positive correlation with ash, crude fat, and total material ingredients. It showed a significant positive correlation with total sugar. Conclusion The accumulation of GA closely relates to the distribution of crude fiber, crude fat, ash, and total sugar. The Mo at appropriate concentration could stimulate the formation of sugar and crude fat, and then promote the secondary metabolism of G. uralensis. All above could lead to the formation and accumulation of GA.