| 甘西鼠尾草愈伤组织诱导、增殖及分化研究 |
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| 引用本文： | 王萌,张丽,谢显莉,张利. 甘西鼠尾草愈伤组织诱导、增殖及分化研究[J]. 中草药, 2011, 42(6): 1206-1209 |
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| 作者姓名： | 王萌 张丽 谢显莉 张利 |
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| 作者单位： | 1. 四川农业大学生命科学与理学院,四川雅安,625014
2. 四川省中江县农业局,四川德阳,618100 |
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| 基金项目： | 四川省青年科技基金，四川省科技厅资助项目，四川省科技厅支撑项目，四川省科技厅产学研创新联盟合作项目 |
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| 摘 要： | 目的考察不同质量浓度激素组合对甘西鼠尾草愈伤组织诱导、增殖及分化的影响。方法以甘西鼠尾草叶片为外植体,采用正交试验研究2,4-D、NAA、6-BA、KT对愈伤组织诱导及增殖的影响,并探讨适宜愈伤组织分化的培养条件。结果以诱导率和相对生长速率为指标,6-BA和2,4-D均对甘西鼠尾草细胞脱分化和细胞增殖具有显著影响,愈伤组织诱导的最佳激素组合为2,4-D 0.5 mg/L+NAA 1.0 mg/L+6-BA 0.5 mg/L,增殖的最佳激素组合为2,4-D 0.5 mg/L+6-BA 2.0 mg/L+KT 0.1 mg/L,MS+NAA 0.5 mg/L+6-BA 1.0 mg/L的培养基有利于不定芽的分化,而1/2 MS+IBA 1.0 mg/L的培养基则有利于不定根的分化。结论本实验所建立的组织培养体系,适宜甘西鼠尾草的植株再生。
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| 关 键 词： | 甘西鼠尾草 愈伤组织 正交试验 激素 分化 |
| 收稿时间： | 2010-11-26 |
Induction, proliferation, and differentiation on callus culture of Salvia przewalskii |
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| WANG Meng,ZHANG Li,XIE Xian-li and ZHANG Li. Induction, proliferation, and differentiation on callus culture of Salvia przewalskii[J]. Chinese Traditional and Herbal Drugs, 2011, 42(6): 1206-1209 |
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| Authors: | WANG Meng ZHANG Li XIE Xian-li ZHANG Li |
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| Affiliation: | WANG Meng1,ZHANG Li1,XIE Xian-li2,ZHANG Li1 1.College of Biology and Science,Sichuan Agricultural University,Yaan 625014,China 2.Bureau of Agriculture in Zhongjiang County of Sichuan Province,Deyang 618100,China |
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| Abstract: | Objective To investigate the effects of different concentrations of hormonal combinations on induction,proliferation,and differentiation in Salvia przewalskii callus culture.Methods Using sterile leaves of S.przewalskii as explants,the different concentrations of 2,4-D,NAA,6-BA,and KT on induction,proliferation of callus were optimized by orthogonal test.Meanwhile the suitable hormonal combinations for differentiation of callus were studied.Results The results showed that taking the inductive rate and the r... |
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| Keywords: | Salvia przewalskii Maxim. callus orthogonal test hormone differentiation |
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